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Yspvsildi

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The YSPVSILDI is a laboratory equipment designed for specific scientific applications. It serves a core function within its intended use. No further details can be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Tnf α fitc, by BD (2 mentions) Ack lysing buffer, by Thermo Fisher Scientific (2 mentions) Ifn γ pe, by BD (2 mentions) Data analysis software, by FlowJo (2 mentions) Hank s medium, by Corning (2 mentions) Evknwmtdtl, by BD (2 mentions) Cytofix cytoperm kit, by BD (2 mentions) Golgiplug, by BD (2 mentions) Cd8 pacific blue, by BD (2 mentions)

2 protocols using yspvsildi

1

Intracellular Cytokine Staining of Gag-Specific T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleens were harvested in Hank’s medium (Corning Cellgro) and cells were strained through a 70 μm filter for resuspension in complete RPMI [cRPMI: 10% FBS, penicillin (100 units/mL), streptomycin (100 μg/mL), 2 mM L-glutamine]. Red blood cells were lysed with ACK Lysing Buffer (Gibco) and splenocytes were resuspended in cRPMI at a concentration of 2×107 cells/mL. Cells were plated at 1×106 cells/well and incubated for 5 h in the presence of Golgi Plug (BD Biosciences) and 1 μg of one of five different immunodominant ZM96 Gag CD8 epitopes: LRSLYNTV (LRS8), VIPMFTAL (VIP8), AMQMLKDT (AMQ8), YSPVSILDI (YSP9), EVKNWMTDTL (EVK10) (synthesized by GenScript and resuspended according to manufacturer’s protocol). Cells were stained with fluorescently conjugated antibodies: CD8-Pacific Blue, TNFα-FITC, and IFNγ-PE (BD Biosciences). Fixation and permeabilization was performed with a Cytofix/Cytoperm Kit (BD Biosciences) with final resuspension in FACS Buffer (1% FBS in 1× PBS). Samples were analyzed by flow cytometry on an LSR Fortessa. Data was analyzed using FlowJo Data Analysis Software (FlowJo, LLC; Ashland, OR).
Meador L.R., Kessans S.A., Kilbourne J., Kibler K.V., Pantaleo G., Roderiguez M.E., Blattman J.N., Jacobs B.L, & Mor T.S. (2017). A heterologous prime-boosting strategy with replicating Vaccinia virus vectors and plant-produced HIV-1 Gag/dgp41 virus-like particles. Virology, 507, 242-256.
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2

Intracellular Cytokine Staining of Gag-Specific T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Spleens were harvested in Hank’s medium (Corning Cellgro) and cells were strained through a 70 μm filter for resuspension in complete RPMI [cRPMI: 10% FBS, penicillin (100 units/mL), streptomycin (100 μg/mL), 2 mM L-glutamine]. Red blood cells were lysed with ACK Lysing Buffer (Gibco) and splenocytes were resuspended in cRPMI at a concentration of 2×107 cells/mL. Cells were plated at 1×106 cells/well and incubated for 5 h in the presence of Golgi Plug (BD Biosciences) and 1 μg of one of five different immunodominant ZM96 Gag CD8 epitopes: LRSLYNTV (LRS8), VIPMFTAL (VIP8), AMQMLKDT (AMQ8), YSPVSILDI (YSP9), EVKNWMTDTL (EVK10) (synthesized by GenScript and resuspended according to manufacturer’s protocol). Cells were stained with fluorescently conjugated antibodies: CD8-Pacific Blue, TNFα-FITC, and IFNγ-PE (BD Biosciences). Fixation and permeabilization was performed with a Cytofix/Cytoperm Kit (BD Biosciences) with final resuspension in FACS Buffer (1% FBS in 1× PBS). Samples were analyzed by flow cytometry on an LSR Fortessa. Data was analyzed using FlowJo Data Analysis Software (FlowJo, LLC; Ashland, OR).
Meador L.R., Kessans S.A., Kilbourne J., Kibler K.V., Pantaleo G., Roderiguez M.E., Blattman J.N., Jacobs B.L, & Mor T.S. (2017). A heterologous prime-boosting strategy with replicating Vaccinia virus vectors and plant-produced HIV-1 Gag/dgp41 virus-like particles. Virology, 507, 242-256.
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