Streptococcus salivarius subsp. thermophilus DSM 20259 (synonym S. thermophilus) was obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ; Braunschweig, Germany). L. plantarum WCFS1, isolated from human saliva as described by Kleerebezem et al. [21 (link)], was originally obtained from NIZO Food Research (Ede, The Netherlands) and maintained in the culture collection of the Norwegian University of Life Sciences, Ås, Norway. Escherichia coli DH5α (New England Biolabs, Frankfurt am Main, Germany) was used in the transformation experiments involving the subcloning of DNA fragments. S. thermophilus and L. plantarum were cultivated in M-17 broth and in MRS media, respectively, at 37°C without agitation. E. coli NEB5α (New England Biolabs, Frankfurt am Main, Germany) was grown at 37°C in Luria–Bertani (LB) medium with shaking at 120 rpm. When needed, erythromycin was supplemented to media in concentrations of 5 μg/mL for Lactobacillus or 200 μg/mL for E. coli, whereas ampicillin was used at 100 μg/mL for E. coli.
Dsm 20259
Manufactured by Leibniz Institute DSMZ
Sourced in Germany
The DSM 20259 is a laboratory equipment item available from the Leibniz Institute DSMZ. It is a device designed for scientific research purposes. The core function of this equipment is to provide a controlled environment for the cultivation and study of microorganisms. Further details on the specific intended use or capabilities of this product are not available.
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Cultivating Diverse Bacterial Strains
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Streptococcus salivarius subsp. thermophilus DSM 20259 (synonym S. thermophilus) was obtained from the German Collection of Microorganisms and Cell Cultures (DSMZ; Braunschweig, Germany). L. plantarum WCFS1, isolated from human saliva as described by Kleerebezem et al. [21 (link)], was originally obtained from NIZO Food Research (Ede, The Netherlands) and maintained in the culture collection of the Norwegian University of Life Sciences, Ås, Norway. Escherichia coli DH5α (New England Biolabs, Frankfurt am Main, Germany) was used in the transformation experiments involving the subcloning of DNA fragments. S. thermophilus and L. plantarum were cultivated in M-17 broth and in MRS media, respectively, at 37°C without agitation. E. coli NEB5α (New England Biolabs, Frankfurt am Main, Germany) was grown at 37°C in Luria–Bertani (LB) medium with shaking at 120 rpm. When needed, erythromycin was supplemented to media in concentrations of 5 μg/mL for Lactobacillus or 200 μg/mL for E. coli, whereas ampicillin was used at 100 μg/mL for E. coli.
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