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5 protocols using 3 mercapto 1 propanol

1

Synthesis of Metal Nanoparticles

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Ethylene glycol, ethanol, and toluene were purchased from Samchun Chemicals (Republic of Korea). Poly(styrene-ethylene-butylene-styrene) (SEBS (H1041)) elastomer was obtained from Asahi Kasei (Japan). Silver nitrate, copper (II) chloride, poly(vinylpyrrolidone) (PVP, Mw, ~ 40 k and ~ 1,300 k), 1-Propanethiol, and 3-Mercapto-1-propanol were purchased from Sigma Aldrich (USA).
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2

Polycrystalline Gold Electrode Functionalization

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Polycrystalline
gold
electrodes (PGEs) of 2 mm diameter were purchased from IJ Cambria
Scientific Ltd. (Llanelli, UK). 3-Mercapto-1-propanol (MCP) was obtained
from Sigma-Aldrich (Dorset, UK). All other chemicals required were
purchased from Acros Organics (Thermo Fisher Scientific Ltd.) (Geel,
Belgium).
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3

Functionalization and Characterization of TFV

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TFV was purchased from Ark Pharm, Inc. (Libertyville, IL). Bovine serum albumin (BSA), keyhole limpet hemocyanin (KLH), sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC), 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), Pierce Protein G agarose beads, CarboxyLink Coupling Gel, Whatman chromatography paper, and pico chemiluminescent substrate were purchased from ThermoFisher (Waltham, MA). Amersham Protran nitrocellulose was purchased from GE Healthcare Life Sciences (Pittsburgh, PA). Anti-rabbit antibody conjugated to horseradish peroxidase (HRP) was purchased from GE Healthcare (Chicago, IL). Goat anti-rabbit antibody was purchased from Abcam (Cambridge, MA). N,N′-Dicyclohexylcarbodiimide (DCC), pyridine, 3-mercapto-1-propanol, silica gel Davisil grade 643, Hi-Flow Plus HF180 nitrocellulose sheets, adenosine monophosphate, Tween 20, sucrose, adenosine monophosphate (AMP), and all solvent and buffers were purchased from Sigma-Aldrich (St. Louis, MO). A 40nm InnovaCoat Gold Conjugation Kit was purchased from Innova Biosciences (Babraham, England).
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4

Electrochemical Biosensing Reagent Preparation

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NaCl, KCl, KH2PO4, NaH2PO4, and NaOH were acquired from Fischer Scientific (NJ). H2SO4 was purchased from EMD (USA), and 2 mm gold electrodes, fritted Ag|AgCl electrodes, and platinum wire were from CHInstruments (TX). 6-Mercapto-1-hexanol, 11-mercapto-1-undecanol, 3-mercapto-1-propanol, tris(2-carboethyl)-phosphine hydrochloride (TCEP), cocaine, and murine monoclonal anti-FLAG M2 antibody were obtained from Sigma-Aldrich (MO). Kanamycin monosulfate was purchased from GoldBio.com (MO), and ethanol was obtained from Gold Shield Distributors (CA). 2 and 7/8″ microcloth, 1 μm monocrystalline diamond suspension, and 0.05 μm micropolish alumina powder were obtained from Buehler (IL). All were used as received.
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5

Electrochemical Microheater Sensor Protocol

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Chromium (99.99%) and gold (99.99%) used for the production of the microheater integrated electrode were purchased from Nanoovak (Turkey). Oligonucleotides were purchased from Sigma Aldrich, and the sequences are shown in Table 1. Control measurements (off-chip experiments) were performed using C223AT Screen Printed Electrodes (SPEs) with 1.6 mm gold working area purchased from Metrohm (Herisau, Switzerland). K4[Fe(CN)6]•3H2O (potassium ferrocyanide), K3[Fe(CN)6] (potassium ferricyanide), 3-Mercapto-1-propanol (MCP), tris(2-carboxyethyl)phosphine (TCEP), phosphate buffered saline (PBS), sulfuric acid (H2SO4) were purchased from Sigma Aldrich (USA). A PalmSens-P4 potenstiostat (PalmSens, Houten, Netherlands) was used to carry out EIS measurements.
Table 1. DNA sequences used for the experiment.
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