Proteins from cultured tumor cells were resolved using SDS-PAGE and transferred onto polyvinylidene fluoride membranes using electroblotting. The membranes were incubated with primary antibodies, all diluted to 1:1000 (Cell Signaling Technology), at 4 °C overnight. β-actin (1:200; Santa Cruz Biotechnology, Dallas, TX) was used as the loading control. The membranes were incubated with HRP-conjugated goat anti-rabbit secondary Abs (1:2500; Sigma-Aldrich, St. Louis, MO) at 25 °C for 1 h. Bound Abs were visualized using a chemiluminescence detection system. Protein levels were calculated as the ratio of the target protein value to the β-actin value.
Hrp conjugated goat anti rabbit secondary abs
Manufactured by Merck Group
HRP-conjugated goat anti-rabbit secondary Abs is a laboratory reagent used in various immunoassays and immunohistochemistry techniques. It is composed of horseradish peroxidase (HRP) enzyme conjugated to goat-derived antibodies that specifically recognize and bind to rabbit primary antibodies. This product serves as a detection tool, amplifying the signal generated from the primary antibody-antigen interaction.
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Lab products found in correlation
3 protocols using hrp conjugated goat anti rabbit secondary abs
1
Western Blot Analysis of Tumor Proteins
2
Immunoblotting of Cultured Macrophages
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Proteins from cultured macrophages were resolved using SDS-PAGE and transferred onto polyvinylidene fluoride membranes using electroblotting. The membranes were incubated with primary antibodies, all diluted to 1:1000 (Cell Signaling Technology), at 4˚C overnight. GAPDH (1:200; Santa Cruz Biotechnology, Dallas, TX) was used as the loading control. The membranes were incubated with HRP-conjugated goat anti-rabbit secondary Abs (1:2500; Sigma-Aldrich, St. Louis, MO) at 25˚C for 1 h. Bound Abs were visualized using a chemiluminescence detection system. Protein levels were calculated as the ratio of the target protein value to the GAPDH value.
3
Western Blot Protein Quantification
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Proteins from cultured macrophages were resolved using SDS-PAGE and transferred onto polyvinylidene uoride membranes using electroblotting. The membranes were incubated with primary antibodies, all diluted to 1:1000 (Cell Signaling Technology), at 4˚C overnight. GAPDH (1:200; Santa Cruz Biotechnology, Dallas, TX) was used as the loading control. The membranes were incubated with HRP-conjugated goat anti-rabbit secondary Abs (1:2500; Sigma-Aldrich, St. Louis, MO) at 25˚C for 1 h. Bound Abs were visualized using a chemiluminescence detection system. Protein levels were calculated as the ratio of the target protein value to the GAPDH value.
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