Total RNA was extracted from mouse amygdalar tissues with Trizol reagent (Invitrogen) and was quantified by AceQ Universal SYBR qPCR Master Mix Kit (Vazyme, Q511) under the following conditions: (95°C for 10 min; 40 cycles of 95°C for 15 s, 65°C for 1 min, 95°C for 15 s; 60°C for 1 min, 95°C for 1 s). Primers sequences (Sangon Biotech) for amplification were follows CLDN5 (Forward 5′-TTTCTTCTATGCGCAGTTGG-3′, Reverse GCAGTTTGGTGCCTACTTCA-3′), GAPDH (Forward 5′-AACTTTGGCATTGTGGAAGG-3′, Reverse 5′-GGATGCAGGGATGATGTTCT-3′).
Primers sequences
Manufactured by Sangon
Sourced in United States
Primers are short, synthetic DNA sequences designed to serve as the starting point for DNA replication during various molecular biology techniques, such as polymerase chain reaction (PCR). They provide the necessary template for DNA polymerase to initiate the amplification process.
Automatically generated - may contain errors
2 protocols using primers sequences
1
Quantitative Analysis of CLDN5 Expression
2
Aluminum Chloride Protocol for RT-PCR
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aluminum chloride (AlCl3) was purchased from Sigma Aldrich Hyderabad; primers sequences obtained from Shanghai Sangon Biotech; RT-PCR kits by Promega, USA and Trizol reagent from Invitrogen, Germany.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!