Gabaar γ2

Manufactured by Santa Cruz Biotechnology

GABAAR-γ2 is a protein subunit of the GABA-A receptor, which is a ligand-gated ion channel that mediates the majority of the fast inhibitory neurotransmission in the central nervous system. The GABAAR-γ2 subunit plays a crucial role in the assembly and function of the GABA-A receptor.

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Lab products found in correlation

Enhanced chemiluminescence solution, by Thermo Fisher Scientific (1 mentions) Flag tag (flag), by Merck Group (1 mentions) β actin, by Merck Group (1 mentions) Imagequant las 4000 mini molecular imaging system, by GE Healthcare (1 mentions) Las 4000, by Cytiva (1 mentions) Synaptophysin, by Merck Group (1 mentions) Gephyrin, by Santa Cruz Biotechnology (1 mentions)

2 protocols using gabaar γ2

Western Blot Analysis of GABA Receptors

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Protein samples from cultured HEK-293T cells were separated using sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride filters. The filters were incubated overnight at 4 °C with appropriate antibodies. Secondary antibodies conjugated to horseradish peroxidase were added to the filters and then visualized in the enhanced chemiluminescence solution (Thermo Scientific, USA). The visualization was performed via the ImageQuant LAS 4000 mini Molecular Imaging System (GE Healthcare Life Sciences, USA), and the Image J software (NIH, USA) was used for the analysis of band intensity. The antibodies used were as follows: β-actin (1:1000; Chemicon, Cat # MAB1501), FLAG (1:4000; Sigma-Aldrich/Merck Millipore, Cat # F1804), GABA_AR-α5 (1: 1000; Chemicon, Cat # AB9678), and GABA_AR-γ2 (1:100; Santa Cruz, Cat # sc-101963).

Fan H.R., Du W.F., Zhu T., Wu Y.J., Liu Y.M., Wang Q., Wang Q., Gu X., Shan X., Deng S., Zhu T., Xu T.L., Ge W.H., Li W.G, & Li F. (2018). Quercetin Reduces Cortical GABAergic Transmission and Alleviates MK-801-Induced Hyperactivity. EBioMedicine, 34, 201-213.

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Antibody Panel for Neuronal Markers

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The following antibodies to the listed proteins were obtained from commercial sources (abbreviation, host and type, and supplier in parentheses): glutamic acid decarboxylase (GAD, mouse monoclonal, MBL International, Woburn, MA); class-III-β-tubulin (mouse monoclonal, R & D Systems, Minneapolis, MN); GABA-A-receptor γ2-subunit (GABA-A-Rγ2, goat polyclonal, Santa Cruz Biotechnology); glycine receptor α1/2-subunits (GlyRα1/2, rabbit polyclonal, Thermo Fisher Scientific, Waltham, MA); gephyrin (mouse monoclonal, Santa Cruz Biotechnology); glycine transporter 2 (GlyT2, rabbit polyclonal, Santa Cruz Biotechnology); synaptophysin (rabbit polyclonal, Millipore, Temecula, CA); and non-phosphorylated neurofilament protein (mouse monoclonal, Covance, Emeryville, CA).

Koeppen A.H., Ramirez L., Becker A.B., Feustel P.J, & Mazurkiewicz J.E. (2015). Friedreich Ataxia: Failure of GABA-ergic and Glycinergic Synaptic Transmission in the Dentate Nucleus. Journal of neuropathology and experimental neurology, 74(2), 166-176.

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