On D28, TGF-β1 and ITS SPIO-labeled or not labeled sponges (0–12.5–25–50–100 and 200 µg Fe/mL) were placed in 24 well plates. MR Imaging of MSC-seeded sponges was performed on a clinical 3T MRI system (General Electric) using multi-slice multi-echo 2D T2 mapping sequences. MRI experiments were conducted with the following acquisition parameters: multi-slice multi-echo T2 mapping sequences (180° flip angle), TR/TE: 1500 ms, 8 echoes (from 10 to 160 ms), slice thickness:1 mm, NEX: 3 and acquisition time: 32 min. The MR images were obtained using a matrix size of 288×224 reconstructed by interpolation in 512×512 with a resolution of 0.234 mm (pixel spacing). Regions of interest of the appropriate size were chosen within the samples. Determination of T2 values was obtained by the reconstruction of T2 map from 8 echoes sequences by using of FuncTool Software (GE Medical systems, Europe) and especially the Cartigram software package.
3t mri system
Manufactured by GE Healthcare
Sourced in United States
The 3T MRI system is a magnetic resonance imaging (MRI) scanner that operates at a field strength of 3 Tesla. It is designed to capture high-resolution images of the body's internal structures and functions. The 3T MRI system utilizes powerful magnets and radio frequency waves to generate detailed images, allowing healthcare professionals to diagnose and monitor various medical conditions.
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4 protocols using 3t mri system
1
MRI Evaluation of SPIO-Labeled MSC Sponges
2
HIFU Hemiablation and Prostate Cancer Outcomes
3
Comprehensive MRI Biomarkers for MS Prediction
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We acquired brain MRI scans on a 3-T MRI system (GE Medical Systems, Milwaukee, WI) using an eight-channel receive-only head radiofrequency (RF) coil. Detailed descriptions of the MRI protocols and analysis pipelines are shown in Figure 2 and Supplementary Material .
MRI measures that entered the predictive modelling were selected to capture as many aspects of MS damage as possible in a single scan session. We considered lesional and non-lesional damage, functional responses and cerebral blood flow (CBF). For all MRI modalities, we extracted measures from predefined regions of interest (ROIs; ROI-based MRI measures); functional magnetic resonance imaging (fMRI) measures were defined in task-relevant ROIs. For all MRI modalities, we also extracted measures from areas of significant correlations with home-practice outcome (outcome-relevant MRI measures;Table 2 ) for use in the models of prediction.
MRI measures that entered the predictive modelling were selected to capture as many aspects of MS damage as possible in a single scan session. We considered lesional and non-lesional damage, functional responses and cerebral blood flow (CBF). For all MRI modalities, we extracted measures from predefined regions of interest (ROIs; ROI-based MRI measures); functional magnetic resonance imaging (fMRI) measures were defined in task-relevant ROIs. For all MRI modalities, we also extracted measures from areas of significant correlations with home-practice outcome (outcome-relevant MRI measures;
4
Multi-Modal Pulmonary Imaging Protocol
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Anatomical proton ( 1 H) and hyperpolarised 3 He ventilation magnetic resonance images were acquired within 5 min of one another using a 3T MRI system (General Electric Healthcare, Milwaukee, WI, USA), as previously described [24] . Subjects were instructed to inhale 1.0 L of gas (100% N 2 for 1 H MRI and a 3 He/N 2 mixture for 3 He MRI) from FRC, and coronal images were acquired under breath-hold conditions. He gas was polarised to 40% (Polarean; HeliSpin, Durham, NC, USA) and diluted with nitrogen gas to 5 mL per kilogram of body weight.
Thoracic computed tomography (CT) volumes were acquired with a 64-slice Lightspeed VCT system (General Electric Healthcare, Milwaukee, WI, USA) with subjects in breath-hold after inhalation of 1.0 L of nitrogen gas from FRC as previously described [25] . Using the manufacturer's settings and the ImPACT CT patient dosimetry calculator (based on the UK Health Protection Agency NRPB-SR250 software), the total effective dose was 1.8 mSv.
Thoracic computed tomography (CT) volumes were acquired with a 64-slice Lightspeed VCT system (General Electric Healthcare, Milwaukee, WI, USA) with subjects in breath-hold after inhalation of 1.0 L of nitrogen gas from FRC as previously described [25] . Using the manufacturer's settings and the ImPACT CT patient dosimetry calculator (based on the UK Health Protection Agency NRPB-SR250 software), the total effective dose was 1.8 mSv.
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