Imaging was performed on Nikon Eclipse NI‐E microscopes equipped with CFI60 Plan Apochromat Lambda 40× Objectives; C‐FL AT GFP/FITC Long‐Pass Filter Sets; Hamamatsu ORCA‐Flash 4.0 cameras; H101F—ProScan III Open Frame Upright Motorized H101F Flat Top Microscope Stages; and 64‐bit imaging workstations running NIS Elements software (Nikon; Melville, NY). For the reliability studies, these systems were programmed to automatically capture sets of 15 × 15 stitched images containing up to 5,000 sperm. For the acrosome‐exocytosis studies, the C‐FL AT GFP/FITC Long‐Pass Filter Set was modified to have a band‐pass emission filter (ET535/50M). A C‐FL At Cy5/Fluor 647/Draq5 Filter set in combination with a ND16 filter were employed to visualize the Alexa Fluor 647.
H101f proscan 3 open frame upright motorized h101f flat top microscope stages
Manufactured by Nikon
The H101F—ProScan III Open Frame Upright Motorized H101F Flat Top Microscope Stages is a motorized microscope stage designed for use with upright microscopes. It features a flat top design and motorized controls for precise positioning and movement of the sample.
Automatically generated - may contain errors
3 protocols using h101f proscan 3 open frame upright motorized h101f flat top microscope stages
1
High-throughput Sperm Imaging and Analysis
2
Nikon Eclipse NI‐E Microscope Imaging
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Imaging was performed on Nikon Eclipse NI‐E microscopes equipped with CFI60 Plan Apochromat Lambda 40x Objectives; C‐FL AT GFP/FITC Long‐Pass Filter Sets; Hamamatsu ORCA‐Flash 4.0 cameras; H101F—ProScan III Open Frame Upright Motorized H101F Flat Top Microscope Stages; and 64‐bit imaging workstations running NIS Elements software (Nikon; Melville, NY).
3
Nikon Microscope Imaging Workflow
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Imaging was performed on Nikon Eclipse NI‐E microscopes equipped with CFI60 Plan Apochromat Lambda 20× Objectives; C‐FL AT GFP/FITC Long‐Pass Filter Sets; Hamamatsu ORCA‐Flash 4.0 cameras; H101F – ProScan III Open Frame Upright Motorized H101F Flat Top Microscope Stages; and 64‐bit imaging workstations running NIS Elements software (Nikon; Melville NY).
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