Zenon alexa fluor 488 human igg labeling kit

Manufactured by Thermo Fisher Scientific

The Zenon Alexa Fluor 488 Human IgG Labeling Kit is a product designed for the fluorescent labeling of human immunoglobulin G (IgG) antibodies. It utilizes the Alexa Fluor 488 fluorophore to label the IgG molecules, enabling their detection and visualization in various applications.

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Lab products found in correlation

Nucblue live readyprobes reagent, by Thermo Fisher Scientific (1 mentions) Maleimidocaproyl val cit pabc monomethyl auristatin e vcmmae, by MedChemExpress (1 mentions) Prolong diamond antifade mountant, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Zenon labeling kit Alexa Fluor 488 Zenon kit Alexa 488 Alexa Fluors

2 protocols using zenon alexa fluor 488 human igg labeling kit

Chromatographic Purification and Labeling Protocol

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The chromatographic columns
HiTrap MabSelect
SuRe and HiTrap Desalting with Sephadex G-25 resin were obtained from
GE Healthcare (UK). Reagents for microscopy CellLight Early Endosomes-RFP,
BacMam 2.0 (#C10587), Zenon Alexa Fluor 488 Human IgG Labeling Kit
(#Z25402), and NucBlue Live ReadyProbes Reagent (#R37605) were purchased
from Thermo Fisher Scientific (Waltham, MA). Conjugation reagents
tris(2-carboxyethyl) phosphine (TCEP) pH 7.0 (#646547) was obtained
from Merck (Darmstadt, Germany), maleimidocaproyl-Val-Cit-PABC-monomethyl
auristatin E (vcMMAE) (#HY-15575, MedChem Express).

Jendryczko K., Rzeszotko J., Krzyscik M.A., Kocyła A., Szymczyk J., Otlewski J, & Szlachcic A. (2022). Drug Conjugation via Maleimide–Thiol Chemistry Does Not Affect Targeting Properties of Cysteine-Containing Anti-FGFR1 Peptibodies. Molecular Pharmaceutics, 19(5), 1422-1433.

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Intracellular Trafficking of Antibody-Drug Conjugates

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Cells were seeded on 24-well plates with collagen1-coated cover glasses at 2.5 × 10⁵ cells and precultured. The cells were treated with Alexa-488-labeled T-DM1 or IgG (labeled using Zenon Alexa Fluor 488 Human IgG Labeling Kit [Thermo Fisher Scientific]) and incubated for 5 or 24 h. After washes, the cells were fixed with 4% paraformaldehyde for 15 min at 37 °C. The mountant used was ProLong Diamond Antifade Mountant (Thermo Fisher Scientific). Fluorescence microscopy at 60 × magnification was used to visualize the cells.

Yamashita-Kashima Y., Shu S., Osada M., Fujimura T., Yoshiura S., Harada N, & Yoshimura Y. (2020). Combination efficacy of pertuzumab and trastuzumab for trastuzumab emtansine-resistant cells exhibiting attenuated lysosomal trafficking or efflux pumps upregulation. Cancer Chemotherapy and Pharmacology, 86(5), 641-654.

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