Blood samples were centrifuged at 3000 revolutions per minute at 4 °C for 10 min and frozen at −80 °C before further processing. The concentrations of 19 cytokines, including interferon-inducible T cell alpha chemoattractant (ITAC), GM-CSF, fractalkine, IFN-γ, IL-10, macrophage inflammatory protein (MIP)-3α, IL-12p70, IL-13, IL-17A, IL-1β, IL-2, IL-4, IL-23, IL-5, IL-6, IL-7, IL-8, MIP-1β, and TNF-α, were detected in plasma using a human high-sensitivity T cell magnetic bead panel (Millipore, Billerica, MA, USA, HSTCMAG-28SK) with a Luminex 200 multiplex immunoassay system based on the manufacturer’s instructions. Milliplex Analyst 5.1 software (EMD Millipore, Billerica, MA) was used with a cubic curve fitting to calculate the cytokine concentrations of the samples. All samples were run in duplicate, and the interassay coefficients of variability were <20%.
Luminex 200 multiplex immunoassay system
Manufactured by Merck Group
Sourced in United States
The Luminex 200 is a multiplex immunoassay system that allows for the simultaneous detection and quantification of multiple analytes in a single sample. The system utilizes proprietary Luminex xMAP technology, which is based on color-coded magnetic beads coated with specific antibodies or ligands. This enables the rapid and efficient analysis of a wide range of biological molecules, including proteins, peptides, and nucleic acids.
Automatically generated - may contain errors
2 protocols using luminex 200 multiplex immunoassay system
1
Cytokine Profiling in Plasma Samples
2
Plasma Cytokine Profiling in Patients
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The patients provided blood samples at baseline, day 13 and day 26, and HCs provided only a single blood sample. Blood samples were collected into EDTA tubes between 8:00 and 10:00 AM after an overnight fast. Tubes were immediately stored at + 4°C and then centrifuged (3000 rpm/min at + 4°C) for 10 minutes within 1 hour. Plasma was obtained and stored at - 80°C.
Plasma in ammatory cytokine levels were detected using the Human High Sensitivity T Cell Magnetic Bead Panel (Millipore, Billerica, MA, USA, HSTCMAG-28SK) performed with a Luminex 200 multiplex immunoassay system based on the manufacturer's instructions. Nineteen cytokines, including interferoninducible T cell alpha chemoattractant (ITAC), granulocyte-macrophage colony stimulating factor (GM-CSF), fractalkine, interferon (IFN)-γ, IL-10, macrophage in ammatory protein (MIP)-3a, IL-12P70, IL-13, IL-17A, IL-1β, IL-2, IL-4, IL-23, IL-5, IL-6, IL-7, IL-8, MIP-1β, and TNF-α, were quanti ed twice. The detailed detection process and interassay coe cients of variability have been described in our previous studies [27] (link).
Plasma in ammatory cytokine levels were detected using the Human High Sensitivity T Cell Magnetic Bead Panel (Millipore, Billerica, MA, USA, HSTCMAG-28SK) performed with a Luminex 200 multiplex immunoassay system based on the manufacturer's instructions. Nineteen cytokines, including interferoninducible T cell alpha chemoattractant (ITAC), granulocyte-macrophage colony stimulating factor (GM-CSF), fractalkine, interferon (IFN)-γ, IL-10, macrophage in ammatory protein (MIP)-3a, IL-12P70, IL-13, IL-17A, IL-1β, IL-2, IL-4, IL-23, IL-5, IL-6, IL-7, IL-8, MIP-1β, and TNF-α, were quanti ed twice. The detailed detection process and interassay coe cients of variability have been described in our previous studies [27] (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!