Log In Sign up
The largest database of trusted experimental protocols

Tmr in situ cell death detection kit

Manufactured by Merck Group
Visit Supplier

The TMR In Situ Cell Death Detection Kit is a laboratory equipment product designed to detect and analyze cell death in biological samples. The kit utilizes terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) technology to identify and quantify apoptotic cells. The core function of the kit is to provide a reliable and standardized method for the assessment of cell death in various experimental and research applications.

Automatically generated - may contain errors

Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (2 mentions) 4 6 diamidino 2 phenylindole (dapi), by Biotium (1 mentions) Axio scan z1, by Zeiss (1 mentions) Orca flash 4, by Hamamatsu Photonics (1 mentions) Volocity, by PerkinElmer (1 mentions)

Spelling variants of this product

In Situ Cell Death Detection Kit (164 citations) Cell Death Detection Kit (6 citations) TMR Red in situ cell death detection kit (4 citations)

2 protocols using tmr in situ cell death detection kit

1

TUNEL Assay with DAPI Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
TUNEL was performed using a standard kit according to the manufacturer’s protocol (TMR In Situ Cell Death Detection Kit, Sigma-Aldrich, #12156792910) in combination with nuclear staining using DAPI (10 ug/mL; Biotium, Fremont, CA, #40043). Images were captured using a 20x objective. The numbers of TUNEL positive nuclei in each image were counted by a blinded operator.
Chou T.W., Chang N.P., Krishnagiri M., Patel A.P., Lindman M., Angel J.P., Kung P.L., Atkins C, & Daniels B.P. (2021). Fibrillar α-synuclein induces neurotoxic astrocyte activation via RIP kinase signaling and NF-κB. Cell Death & Disease, 12(8), 756.
+ Open protocol
+ Expand
2

Quantifying Cell Death in Inflammatory Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Histologic samples were collected 6 h after TNF injection and evaluated on paraffin sections. Cell death was identified by TUNEL (TMR in situ cell death detection kit; Sigma-Aldrich) and counterstained with DAPI (49,6-diamidino-2-phenylindole; Thermo Fisher Scientific). Micrographs were acquired using an Axioscan Z.1 slide scanner (Carl Zeiss) at •20 magnification and an ORCA Flash4 camera (Hamamatsu Photonics) while illuminated by an HXP 120-V light source. Histologic data were analyzed using Volocity (PerkinElmer) and QuPath (GitHub) software.
Delvaeye T., Wyffels L., Deleye S., Lemeire K., Gonçalves A., Decrock E., Staelens S., Leybaert L., Vandenabeele P, & Krysko D.V. (2018). Noninvasive Whole-Body Imaging of Phosphatidylethanolamine as a Cell Death Marker Using 99mTc-Duramycin During TNF-Induced SIRS. Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 59(7).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!