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T activator cd3 cd28 magnetic beads

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T-Activator CD3/CD28 magnetic beads are a laboratory product designed for the activation and expansion of T cells. The beads are coated with antibodies targeting the CD3 and CD28 receptors on the surface of T cells, which stimulates their proliferation and differentiation.

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Lab products found in correlation

Cyto fast fix perm buffer set, by BioLegend (1 mentions) Brefeldin a, by BioLegend (1 mentions)

Close spelling variants of this product

CD3/CD28 T-activator beads (8 citations) CD3/CD28 activator beads (6 citations) CD3/CD28 beads (82 citations) Magnetic beads (264 citations)

2 protocols using t activator cd3 cd28 magnetic beads

1

TILs Isolation and Activation

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TILs were isolated from filtered tumor tissue single-cell suspensions by treatment with Mouse Pan T (CD90.2) magnetic bead selection (Invitrogen, Waltham, MA, USA). Isolated cells were then cultured in vitro in the presence of T-Activator CD3/CD28 magnetic beads (Invitrogen, Waltham, MA, USA) for 24 h. Brefeldin A (Biolegend, San Diego, CA, USA) was added to the culture 4 h before staining with antibodies against CD8, CD4, and CD3, followed by permeabilization with Cyto-Fast™ Fix/Perm Buffer Set (Biolegend, San Diego, CA, USA) and staining with antibodies against IFNγ.
Barsheshet Y., Voloshin T., Brant B., Cohen G., Koren L., Blatt R., Cahal S., Haj Khalil T., Zemer Tov E., Paz R., Klein-Goldberg A., Tempel-Brami C., Jacobovitch S., Volodin A., Kan T., Koltun B., David C., Haber A., Giladi M., Weinberg U, & Palti Y. (2022). Tumor Treating Fields (TTFields) Concomitant with Immune Checkpoint Inhibitors Are Therapeutically Effective in Non-Small Cell Lung Cancer (NSCLC) In Vivo Model. International Journal of Molecular Sciences, 23(22), 14073.
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2

T-Cell Isolation and Activation

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TILs were isolated by dissociating tumor tissue as described above. Single-cell suspensions were filtered and separated by Mouse Pan T magnetic bead selection (Invitrogen). Isolated cells were then cultured in vitro in the presence of T-Activator CD3/CD28 magnetic beads (Invitrogen) for 24 h.
Voloshin T., Kaynan N., Davidi S., Porat Y., Shteingauz A., Schneiderman R.S., Zeevi E., Munster M., Blat R., Tempel Brami C., Cahal S., Itzhaki A., Giladi M., Kirson E.D., Weinberg U., Kinzel A, & Palti Y. (2020). Tumor-treating fields (TTFields) induce immunogenic cell death resulting in enhanced antitumor efficacy when combined with anti-PD-1 therapy. Cancer Immunology, Immunotherapy, 69(7), 1191-1204.
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