Stable isotope labeled standards

Manufactured by Cambridge Isotopes

Sourced in Germany

Stable isotope labeled standards are reference materials that contain a specific element or compound labeled with a stable isotope. These standards are used to accurately quantify and identify the target analyte in analytical procedures.

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Stable isotopes (7 citations) Stable isotope-labeled internal standards (ISs) (2 citations) Stable isotope-labeled acylcarnitine internal standard (2 citations)

2 protocols using stable isotope labeled standards

Iodoacetamide-Based Labeling of Proteins

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HPE-IAM (β-(4-hydroxyphenyl) ethyl iodoacetamide) was purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Dimethyl sulfoxide (DMSO), Sodium selenite, 2-Mercaptoethanol, Protease Inhibitor Cocktail, CHAPS, Giemsa, May-Grünwald Stain were purchased from Sigma (St. Louis, MO, USA). Fetal bovine serum (FBS) was purchased from EuroClone (Pero (MI), Italy), RPMI 1640 medium, l-glutamine, penicillin/streptomycin from Lonza (Basel, Swiss). Phosphatase Inhibitor Cocktail was purchased from Merck (Darmstadt, Germany).
Stable isotope labeled standards were produced by Cambridge Isotope Laboratories, Inc (Tewksbury, MA, USA).

Czikora Á., Erdélyi K., Ditrói T., Szántó N., Jurányi E.P., Szanyi S., Tóvári J., Strausz T, & Nagy P. (2022). Cystathionine β-synthase overexpression drives metastatic dissemination in pancreatic ductal adenocarcinoma via inducing epithelial-to-mesenchymal transformation of cancer cells. Redox Biology, 57, 102505.

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Metabolite Analysis Using HPLC-Grade Solvents

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HPLC-grade acetonitrile (ACN) and methanol (MeOH) were purchased from Sigma-Aldrich (St. Louis, MO). Ammonium acetate and acetic acid were obtained from Fisher Scientific (Pittsburgh, PA). Deionized water was provided in-house by a Millipore Milli-Q Academic Water Purification System (Billerica, MA). Pure metabolite standards in the Arizona Metabolomics Laboratory (AML) were purchased from Sigma-Aldrich and Fisher Scientific. Stable isotope-labeled standards were purchased from Cambridge Isotope Laboratories (Tewksbury, MA).

Shi X., Xi B., Jasbi P., Turner C., Jin Y, & Gu H. (2020). Comprehensive Isotopic Targeted Mass Spectrometry: Reliable Metabolic Flux Analysis with Broad Coverage. Analytical chemistry, 92(17), 11728-11738.

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