Paraffin-embedded blocks were used to obtain several tissue sections that were fixed on adhesive slides for immune staining. Endogenous peroxidase and protein blocking steps were performed after rehydration using hydrogen peroxide and bovine serum albumin (BSA). The tissue slides were washed with Tris buffer several times, followed by the application of primary antibodies (anti-MBP sc-271524, anti-GFAP sc-33673, anti-Olig2 sc-293163, and anti-Bax sc-7480, Santa Cruz Biotechnology, Inc., Heidelberg, Germany) at a dilution of 1:150 for 12 h at 4 °C. After that, washing was done for 2 h at room temperature with a secondary horseradish peroxidase (HRP)-labeled antibody (goat anti-mouse HRP-labeled secondary antibody, Abcam, UK) at a dilution of 1:1000. The color was developed using the DAB Substrate Kit. Negative control slides were obtained by skipping the primary antibody step. Positive expression was quantified as area % using cellSens dimensions (Olympus Software).
Anti mbp sc 271524
Manufactured by Santa Cruz Biotechnology
Sourced in Germany, Palestine, State of
Anti-MBP sc-271524 is a primary antibody product manufactured by Santa Cruz Biotechnology. It is designed for the detection of MBP (Myelin Basic Protein) in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti bax sc 7480, by Santa Cruz Biotechnology (1 mentions)
Anti gfap sc 33673, by Santa Cruz Biotechnology (1 mentions)
Alexa fluor 488 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Paraformaldehyde (pfa), by Merck Group (1 mentions)
Tcs sp8 sted, by Leica (1 mentions)
Anti nav1, by Alomone (1 mentions)
Eclipse ci, by Leica (1 mentions)
Anti olig2, by Merck Group (1 mentions)
Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 594 goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Anti nf200, by Merck Group (1 mentions)
Anti pdgfrα, by Cell Signaling Technology (1 mentions)
2 protocols using anti mbp sc 271524
1
Immunohistochemical Analysis of Neural Markers
2
Quantitative Analysis of Myelination Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Animals were euthanized and perfused with PBS and 4% (w/v) paraformaldehyde (158127, Sigma). Coronal sections (16 μm) of brains blocked by 5% (v/v) goat serum were incubated with primary antibodies and corresponding secondary antibodies. Antibodies used were as follows: anti-MBP (sc271524, Santa Cruz), anti-NF200 (N0142, Sigma), anti-β-APP (51-2700, Invitrogen), anti-Caspr (MABN69, Millipore), anti-Nav1.6 (ASC-009, Alomone, Jerusalem), anti-PDGFRα (3174, Cell Signaling Technology), anti-Olig2 (ab9610, Millipore), Alexa Fluor 594 goat-anti-rabbit IgG (A-11012, Invitrogen), Alexa Fluor 594 goat-anti-mouse IgG (A-11005, Invitrogen), Alexa Fluor 488 goat-anti-rabbit IgG (A-11008, Invitrogen) and Alexa Fluor 488 goat-anti-mouse IgG (A-11001, Invitrogen). Images were obtained by fluorescence microscope (Nikon ECLIPSE Ci) or confocal microscope (TCS SP8 STED, Leica). At least three microscopic fields around the lesion were captured in each section. Three sections, at the sites of anterior, middle and posterior of the hematoma, were chosen for each mouse. Data were analyzed by an investigator who was blinded to the experimental setup.
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