Mbd3l2

Manufactured by Abcam

Sourced in United Kingdom

MBD3L2 is a protein that is involved in the regulation of gene expression. It is a member of the methyl-CpG binding domain (MBD) protein family, which are known to bind to methylated DNA and play a role in epigenetic gene regulation. The primary function of MBD3L2 is to help modulate the accessibility of DNA to transcriptional machinery.

Automatically generated - may contain errors

Lab products found in correlation

Ab1791, by Abcam (1 mentions) β2 ar r11e1, by Santa Cruz Biotechnology (1 mentions) T9026, by Santa Cruz Biotechnology (1 mentions) Gapdh, by Thermo Fisher Scientific (1 mentions) Anti h3k4me3, by Merck Group (1 mentions) Oct3 4, by Santa Cruz Biotechnology (1 mentions) Ssea 4, by Merck Group (1 mentions) Tra 1 60, by Merck Group (1 mentions) Alexa fluor 594 conjugated donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions) Anti h3k4me2, by Merck Group (1 mentions) Anti h3k9me3, by Merck Group (1 mentions) Ssea3, by Merck Group (1 mentions) Zscan4, by Merck Group (1 mentions) Nanog, by Abcam (1 mentions) Anti h3k27me3, by Abcam (1 mentions)

2 protocols using mbd3l2

Antibody Panel for Protein Analysis

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The following antibodies were used: α-Tubulin (DM1A), Sigma-Aldrich T9026; β2-AR (R11E1), Santa Cruz Biotechnology (Dallas, TX, USA) sc-81577, lot#G1117; BRD2, Bethyl Laboratories (Montgomery, TX, USA) A302-582A; BRD3, Bethyl A302-368A; BRD4, Bethyl A301-985A50; GAPDH, Thermo Fisher Scientific TAB1001; Histone H3, Abcam (Cambridge, UK) ab1791; and MBD3L2, Abcam ab107999, lot#GR126890-1; rabbit monoclonal antibody against DUX4 (E14-3) was produced in collaboration with Epitomics (Burlingame, CA, USA) and is described elsewhere [25 (link)].

Campbell A.E., Oliva J., Yates M.P., Zhong J.W., Shadle S.C., Snider L., Singh N., Tai S., Hiramuki Y., Tawil R., van der Maarel S.M., Tapscott S.J, & Sverdrup F.M. (2017). BET bromodomain inhibitors and agonists of the beta-2 adrenergic receptor identified in screens for compounds that inhibit DUX4 expression in FSHD muscle cells. Skeletal Muscle, 7, 16.

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Pluripotent Stem Cell Characterization

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For immunostaining, the primary antibodies used included those against OCT3/4 (1:500, Santa Cruz), SOX2 (1:500, Santa Cruz), NANOG (1:500, Abcam), SSEA3 (1:50, Millipore), SSEA4 (1:50, Millipore), TRA-1–60 (1:50, Millipore), UTF1 (1: 200, Abcam), DPPA3 (1:50, Santa Cruz), ZSCAN4 (1:100, Millipore) and MBD3L2 (1:100, Abcam). The following secondary antibodies were used: Alexa Fluor 594-conjugated donkey anti-mouse IgG (1:500; Invitrogen), fluorescein isothiocyanate (FITC) 488-conjugated donkey anti-rabbit IgG (1:500; Invitrogen), and FITC 488-conjugated donkey anti-goat IgG (1:500; Invitrogen). Anti-H3K4me2 (Millipore), Anti-H3K4me3 (Millipore), Anti-H3K27me3 (Abcam) and Anti-H3K9me3 (Millipore) antibodies were used for ChIP experiments.

Wang Y., Zhao C., Hou Z., Yang Y., Bi Y., Wang H., Zhang Y, & Gao S. (2018). Unique molecular events during reprogramming of human somatic cells to induced pluripotent stem cells (iPSCs) at naïve state. eLife, 7, e29518.

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