Map2 ab

Manufactured by Abcam

Sourced in United Kingdom

MAP2 Ab is a primary antibody that detects the microtubule-associated protein 2 (MAP2) in a variety of species. MAP2 is a widely used neuronal marker that stabilizes and promotes the assembly of microtubules in neuronal dendrites.

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Lab products found in correlation

Anti il 1β ab, by R&D Systems (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Vectashield hardset mounting medium with dapi, by Vector Laboratories (1 mentions) Alexa 555, by Thermo Fisher Scientific (1 mentions) Alex 594, by Thermo Fisher Scientific (1 mentions) Anti myeloperoxidase mpo ab, by Abcam (1 mentions) G3bp1, by Proteintech (1 mentions) Foxg1, by Takara Bio (1 mentions) Elavl4, by Santa Cruz Biotechnology (1 mentions) Tia 1, by Santa Cruz Biotechnology (1 mentions) Axio observer z1, by Zeiss (1 mentions) β tubulin 3, by Merck Group (1 mentions) Glial fibrillary acidic protein (gfap), by Merck Group (1 mentions) Neuronal nuclei (neun), by Merck Group (1 mentions) Nestin, by Merck Group (1 mentions) Synapsin 1, by Merck Group (1 mentions) Ctip2, by Abcam (1 mentions) Satb2, by Abcam (1 mentions) Sox10, by Santa Cruz Biotechnology (1 mentions) Immedge pen, by Vector Laboratories (1 mentions)

2 protocols using map2 ab

Immunofluorescence Staining of Brain Sections

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For immunofluorescence staining, paraffin-embedded brain sections were prepared as described in our previous studies11 (link). The primary antibodies used in the experiments included anti-HMGB1 Ab (R&D Systems Inc., Minneapolis, MN), anti-AQP4 Ab (Abcam Plc, Cambridge, UK), anti-IL-1β Ab (R&D Systems Inc.), anti-microtubule-associated protein 2 (MAP2) Ab (Abcam Plc), anti-glial fibrillary acid protein (GFAP) Ab (Abcam Plc), anti-ionized calcium-binding adaptor molecule 1 (Iba1) Ab (Wako, Osaka, Japan) and anti-myeloperoxidase (MPO) Ab (Abcam Plc). In order to investigate the cellular source as well as the localization of IL-1β, double immunohistochemical staining was carried out with cell marker antibodies including MAP2, GFAP, iba1 or MPO antibodies and an antibody against IL-1β. The sections were then incubated with secondary Abs conjugated with Alexa-488, Alexa-555 or Alex594, which were purchased from Invitrogen (Tokyo, Japan). Finally, the sections were mounted using VECTASHIELD Hard Set Mounting Medium with DAPI (Vector Laboratories Inc., Burlingame, CA) and observed under an LSM 780 confocal microscopic system (Carl Zeiss Inc., Jena, Germany). The counting was performed in a blinded manner.

Wang D., Liu K., Wake H., Teshigawara K., Mori S, & Nishibori M. (2017). Anti-high mobility group box-1 (HMGB1) antibody inhibits hemorrhage-induced brain injury and improved neurological deficits in rats. Scientific Reports, 7, 46243.

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Immunohistochemical Characterization of Neural Tissue

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Slides were thawed to room temperature, and lines drawn to partition the edges of sections on the glass slides using a PAPpen (ImmEdge Pen; Vector Labs H4000). The sections were rehydrated, blocked, permeabilized and immunostained with primary antibodies: PAX6 (BioLegend, 1:200), SOX2 (Santa Cruz, 1:100), β-tubulin III (Sigma, 1:1000), FOXG1 (Takara, 1:500), SOX10 (Santa Cruz, 1:100), Ki67 (Thermo Fisher, 1:200), Nestin (Millipore, 1:200), GFAP (Millipore, 1:400), S100beta (DAKO Potts, 1:400), CTIP2 (Abcam, 1:500), SATB2 (Abcam, 1:50), MAP2AB (Abcam, 1:1000-2000), NeuN (Millipore, 1:200), TBR1(Abcam, 1:500), BRN2 (Millipore, 1:500), MEF2C (Novis, 1:200), AT8 (Invitrogen, 1:1000), PHF1 (gift from Peter Davies,1:1000), Total Tau (DAKO Potts, 1:200), DA9 (gift from Peter Davies, 1:1000), ELAVL4 (Santa Cruz, 1:200), TIA1 (Santa Cruz, 1:100), G3BP1 (Protein Tech, 1:200), VGLUT1 (gift from Susan Morton, Tom Jessell, 1:16,000), Calbindin1 (Swant, 1:10,000), Homer1 (SYSY, 1:250) and Synapsin1 (Millipore, 1:100). Primary antibodies were incubated overnight at 4C, washed three times with PBS and then incubated with corresponding Alexa Fluor conjugated secondary antibody (1:333-1000) for 1 hour at room temperature. Sections were coverslipped and imaged using fluorescence and confocal microscopy (Zeiss AXIO Observer.Z1; Zeiss 780).

Bowles K.R., Silva M.C., Whitney K., Bertucci T., Berlind J.E., Lai J.D., Garza J.C., Boles N.C., Mahali S., Strang K.H., Marsh J.A., Chen C., Pugh D.A., Liu Y., Gordon R.E., Goderie S.K., Chowdhury R., Lotz S., Lane K., Crary J.F., Haggarty S.J., Karch C.M., Ichida J.K., Goate A.M, & Temple S. (2021). ELAVL4, splicing, and glutamatergic dysfunction precede neuron loss in MAPT mutation cerebral organoids. Cell, 184(17), 4547-4563.e17.

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