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Genechip 3 ivt reagent kit

Manufactured by Thermo Fisher Scientific
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The GeneChip 3' IVT Reagent Kit is a laboratory product designed to facilitate the in vitro transcription (IVT) process in gene expression analysis. It provides the necessary reagents to generate amplified and biotinylated cRNA from total RNA samples, which can then be used for hybridization on GeneChip microarray platforms.

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Lab products found in correlation

Nanodrop nd 1000 spectrophotometer, by Thermo Fisher Scientific (2 mentions) Tri reagent, by Merck Group (2 mentions) Genechip scanner 3000, by Thermo Fisher Scientific (2 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (2 mentions) Mouse 430a or mouse 430a 2.0 arrays, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

GeneChip 3′ IVT PLUS Reagent Kit (55 citations) GeneChips (22 citations) GeneChip™ HT 3′IVT PLUS Reagent Kit (6 citations) GeneChip® 3′ Expression Arrays and 3′ IVT PLUS Reagent Kit (3 citations) Human GeneChip 3′ IVT PLUS Reagent Kit (3 citations) GeneChip 3’ IVT Pico Reagent kit (3 citations)

2 protocols using genechip 3 ivt reagent kit

1

Mouse Liver and Adipose Tissue Transcriptome Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three to four biological replicates per experimental condition were carried out for the microarray studies. Total RNA was isolated and purified from mouse liver (sliced into small pieces) and adipose tissue homogenized in Tri-Reagent (Sigma-Aldrich, St. Louis, MO). RNA purity, quantity, and quality were determined using a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Waltham, MA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). The mRNA gene expression microarray experiments were carried out by the Dartmouth Genomics & Molecular Biology Shared Resource using the Mouse 430A or Mouse 430A 2.0 Arrays (Affymetrix, Santa Clara, CA). Approximately 100 ng of total RNA per sample was labeled for each array using the GeneChip 3’ IVT Reagent Kit (Applied Biosystems, Foster City, CA). Labeled RNA was incubated with the arrays and stained using the corresponding kit on the GeneChip Fluidics Station. The arrays were scanned using the Affymetrix GeneChip Scanner 3000.
Rojas I.Y., Moyer B.J., Ringelberg C.S, & Tomlinson C.R. (2020). Reversal of obesity and liver steatosis in mice via inhibition of aryl hydrocarbon receptor and altered gene expression of CYP1B1, PPARα, SCD1, and osteopontin. International journal of obesity (2005), 44(4), 948-963.
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2

Mouse Liver and Adipose Tissue Transcriptome Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Three to four biological replicates per experimental condition were carried out for the microarray studies. Total RNA was isolated and purified from mouse liver (sliced into small pieces) and adipose tissue homogenized in Tri-Reagent (Sigma-Aldrich, St. Louis, MO). RNA purity, quantity, and quality were determined using a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Waltham, MA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA). The mRNA gene expression microarray experiments were carried out by the Dartmouth Genomics & Molecular Biology Shared Resource using the Mouse 430A or Mouse 430A 2.0 Arrays (Affymetrix, Santa Clara, CA). Approximately 100 ng of total RNA per sample was labeled for each array using the GeneChip 3’ IVT Reagent Kit (Applied Biosystems, Foster City, CA). Labeled RNA was incubated with the arrays and stained using the corresponding kit on the GeneChip Fluidics Station. The arrays were scanned using the Affymetrix GeneChip Scanner 3000.
Rojas I.Y., Moyer B.J., Ringelberg C.S, & Tomlinson C.R. (2020). Reversal of obesity and liver steatosis in mice via inhibition of aryl hydrocarbon receptor and altered gene expression of CYP1B1, PPARα, SCD1, and osteopontin. International journal of obesity (2005), 44(4), 948-963.
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