Xenolight rediject d luciferin ultra bioluminescent substrate

Manufactured by PerkinElmer

XenoLight RediJect D-Luciferin Ultra Bioluminescent Substrate is a lab equipment product that serves as a substrate for bioluminescent imaging. It is designed to be used in research applications involving bioluminescent detection and quantification.

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RediJect D-Luciferin Ultra Bioluminescent Substrate XenoLight RediJect d-luciferin substrate XenoLight RediJect D-Luciferin Ultra RediJect D-Luciferin Bioluminescent Substrate XenoLight® RediJect™ D-Luciferin RediJect D-Luciferin Ultra XenoLight d-luciferin substrate D-luciferin bioluminescent substrate RediJect D-Luciferin XenoLight D-Luciferin

2 protocols using xenolight rediject d luciferin ultra bioluminescent substrate

Bioluminescence Imaging of Mice

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Xenogen IVIS imaging system (Caliper Life Sciences) was used for live animal imaging. Mice were anesthetized using 1.5% isofluorane (Piramal Healthcare). XenoLight RediJect D-Luciferin Ultra Bioluminescent Substrate (150 mg/kg body weight; 30 mg/mL in PBS; Perkin Elmer) was injected intraperitoneally and IVIS imaging was performed 7-10 minutes after substrate injection. Whole body bioluminescent signal intensity was determined using IVIS Living Image software v4.3.1 (Caliper Life Sciences), and pseudocolor images overlaid on conventional photographs are shown. Data were analyzed and presented as photon counts per area.

Snyder K.J., Choe H.K., Gao Y., Sell N.E., Braunreiter K.M., Zitzer N.C., Neidemire-Colley L., Kalyan S., Dorrance A.M., Keller A., Mihaylova M.M., Singh S., Sehgal L., Bollag G., Ma Y., Powell B., Devine S.M, & Ranganathan P. (2021). Inhibition of Bromodomain and Extra Terminal (BET) Domain Activity Modulates the IL-23R/IL-17 Axis and Suppresses Acute Graft-Versus-Host Disease. Frontiers in Oncology, 11, 760789.

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Bioluminescent Imaging in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Xenogen IVIS imaging system (Caliper Life Sciences) was used for live animal imaging. Mice were anesthetized using 1.5% isofluorane (Piramal Healthcare). XenoLight RediJect D-Luciferin Ultra Bioluminescent Substrate (150 mg/kg body weight; 30 mg/mL in PBS; Perkin Elmer) was injected intraperitoneally and IVIS imaging was performed 10 minutes after substrate injection. Whole body bioluminescent signal intensity was determined weekly using IVIS Living Image software v4.3.1 (Caliper Life Sciences), and pseudocolor images overlaid on conventional photographs are shown. Data were analyzed and presented as photon counts per area.

Ranganathan P., Ngankeu A., Zitzer N.C., Leoncini P., Yu X., Casadei L., Challagundla K., Reichenbach D.K., Garman S., Ruppert A.S., Volinia S., Hofstetter J., Efebera Y.A., Devine S., Blazar B.R., Fabbri M, & Garzon R. (2017). Serum miR-29a is upregulated in acute Graft versus Host Disease and activates dendritic cells through TLR binding. Journal of immunology (Baltimore, Md. : 1950), 198(6), 2500-2512.

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