For HIF-1α luciferase activity assays, cells with ectopic Myc-Parkin expression or endogenous Parkin knockdown and their controls cells were transfected with the HIF-1α luciferase reporter vector for 12 h36 (link), 61 (link). Cells were then treated with or without hypoxia (1% O2) for 16 h before being harvested for assays. Luciferase reporter assays were performed using the dual luciferase assay kit (Promega). The pRL-null vector expressing renilla luciferase (Promega) was used as an internal control to normalize the transfection efficiency.
Prl null vector expressing renilla luciferase
Manufactured by Promega
The PRL-null vector expressing renilla luciferase is a laboratory tool designed to express the renilla luciferase reporter gene. This vector does not contain the PRL (Prolactin) gene. The primary function of this product is to provide a means to measure and quantify renilla luciferase activity in experimental settings.
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Lab products found in correlation
4 protocols using prl null vector expressing renilla luciferase
1
Hypoxia-Induced HIF-1α Activity Assay
2
TEAD Luciferase Assay for Cell Lines
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For TEAD luciferase activity assays, MGC803 and AGS cells expressing siDUB1 or siControl were transfected with the TEAD luciferase reporter vector for 24 h. Cells were then harvested for assays. Luciferase reporter assays were performed using a dual luciferase assay kit (Promega). The pRL-null vector expressing Renilla luciferase (Promega) was used as the internal control for normalization of the transfection efficiency.
3
TEAD Luciferase Assay for PARK2 Activity
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For TEAD luciferase activity assays, cells with ectopic Flag-PARK2 expression and their control cells were transfected with the TEAD luciferase reporter vector for 24 h. Cells were then harvested for assays. Luciferase reporter assays were performed using the dual luciferase assay kit (Promega). The pRL-null vector expressing renilla luciferase (Promega) was used as an internal control to normalize the transfection efficiency.
4
Measuring TEAD Transcriptional Activity
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For TEAD luciferase activity assays, cells with ectopic HA-PARK2 expression and their control cells were transfected with the TEAD luciferase reporter vector for 24 h. Cells were then harvested for assays. Luciferase reporter assays were performed using the dual luciferase assay kit (Promega). The pRL-null vector expressing renilla luciferase (Promega) was used as an internal control to normalize the transfection e ciency.
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