Neutral Red Cell Proliferation and Total Nitric Oxide Assay Kit were purchased from Beyotime biotechnology (Shanghai, China). Quantitative Analysis Mouse ELISA Kit was ordered from Boster Biological Technology Co., Ltd (Wuhan, China). Immobilon™ Western Chemiluminescent HRP Substrate Kit (WBKL S0100) was purchased from Merck Millipore (CA, USA). Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb (4060), Akt (pan) (C67E7) Rabbit mAb (4691), Phospho-IκBα (Ser32) (14D4) Rabbit mAb (2859), IκBα (44D4) Rabbit mAb (4812), Phospho-NF-kB p65 (Ser536) Rabbit mAb (3033), NF-κB p65 (D14E12) XP® Rabbit mAb (8242), TNFα (D2D4) XP® Rabbit mAb (11948), GAPDH (D4C6R) Mouse mAb (97166) Anti-rabbit IgG (HRP-linked Antibody, 7074) and Anti-mouse IgG (HRP-linked Antibody, 7076) were purchased from Cell Signaling Technology (MA, USA).
Mouse elisa kit
Manufactured by Boster Bio
Sourced in China, United States
Mouse ELISA kits are laboratory equipment used to detect and quantify the presence of specific proteins, hormones, or other analytes in mouse biological samples. These kits provide a standardized and reliable method for conducting enzyme-linked immunosorbent assays (ELISAs) to measure target analytes in a variety of mouse matrices, such as serum, plasma, cell culture supernatants, and tissue homogenates.
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Lab products found in correlation
8 protocols using mouse elisa kit
1
Quantifying Cell Proliferation and Nitric Oxide
2
Biomarker serum levels evaluation
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The serum levels of uPAR, β-CTX, CTX-I, PINP, OCN, ON, and OPN were evaluated by a mouse ELISA kit (Boster, Wuhan, China) according to the operation manual.
3
Measuring IL-6 in Macrophage Cultures
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Culture supernatants collected from peritoneal macrophages and lipopolysaccharide (100 ng/mL)-stimulated peritoneal macrophages were analyzed for interleukin (IL)-6 by a Mouse ELISA Kit (EK0411, Boster Biological Technology, CA, USA), following the manufacturer's instructions.
4
Cytokine Profiling in Mice
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After the behavioural tests, mice were sacrificed with deep anesthesia and blood was collected. The cells’ supernatant was collected after drug intervention for further assays. Serum and culture medium supernatant were collected by centrifugation at 2000 × g and 4 °C for 20 min and stored at −20 or −80 °C. The levels of TNF-α, IL-1β, IL-6, IL-4 and IL-10 were measured using corresponding mouse ELISA kits (Boster) following the manufacturers’ protocol.
5
Mouse Cytokine Profiling in Tumors
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Tumors tissues were isolated on ice, weighed, and homogenized in PBS containing 5 μl protease inhibitor Cocktail (Roche) per mg tissue. Supernatants of tissue homogenate were harvested following centrifugation at 12,000 × g for 20 min at 4 ˚C. The level of mouse TNF-α, IFN-γ, GzmB, IL-2, IL-6, IL-12 was assessed using mouse ELISA kits (Boster, Wuhan, China) following the manufacturer’s instructions.
6
5-HMF Attenuates Inflammatory Responses
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RAW 264.7 cells were seeded in a 12-well plate (3 × 105 cells/each well) and incubated at 37 °C with 5% CO2 for 24 h. After that, the cells were pretreated with 5-HMF at various concentrations (0, 31.5, 63.0 and 126.0 μg/mL) for 6 h and then stimulated with LPS (1 μg/mL) for 18 h. Each medium was collected in a tube and centrifuged at 1000 rpm for 10 min. The expression levels of TNF-α, IL-1β and IL-6 were determined with the mouse ELISA kits (Boster Biological Technology, Wuhan, China) and PGE2 was determined with the mouse ELISA kits (CUSABIO, Shanghai, China) as per each manufacturer’s instructions.
7
Quantitative Evaluation of Cytokine Levels
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The quantitative evaluation of the TNF-α and IL-10 levels in the plasma and BAL were carried out using mouse ELISA kits that were purchased from Boster Biological Technology Co. (CA, USA). The test samples and cytokine standards were added to 96-well plates coated with coating antibody, and the plates were then incubated at 37°C for 90 min. After incubation at 37°C for 30 min, the plates were developed with tetramethyl benzidine at 37°C for 20–25 min. The reaction was stopped by the addition of 100 μl of stop solution. The absorbance was measured using an ELISA reader at 450 nm. The concentrations of TNF-α and IL-10 were calculated according to the standard curve using each of the recombinant cytokines in the ELISA kits [21 (link)].
8
Quantifying Lung Neutrophil Accumulation
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The superior and middle lobes of the right lung were collected and homogenized for the detection of myeloperoxidase (MPO) activity (an indicator of neutrophil accumulation) [24 (link), 31 (link)] following a test kit protocol (Cat. No. A044-1-1, Nanjing Jiancheng Bioengineering Institute, Nanjing, China). Cytokines in BALF and serum were determined with mouse ELISA kits (Boster Biological Technology Co., Ltd., Wuhan, China) for IL-6 (Cat. No. EK0411), TNF-α (Cat. No. EK0527), and ICAM-1 (Cat. No. EK0371).
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