Hematoxylin eosin reagent

Immediately after removal, rat liver portions were fixed for 1 day at room temperature in Carnoy’s fixative (ethanol:chloroform:acetic acid, 6:3:1), then preserved in 70% ethanol. Liver specimens were dehydrated with a graded series of alcohol preparations and embedded in paraffin. Specimens were cut in 7 μm sections using a rotary microtome and mounted on 3-aminopropyltriethoxysilane-coated glass slides. Each section was de-paraffinized in xylene, rehydrated in decreasing concentrations of alcohol in water, and stained with hematoxylin-eosin reagent (Sigma). The slide was then mounted with neutral balsam.

After sacrifice, the distal colon tissues of the mice were immediately fixed with 4% formalin, and they were washed, dehydrated, embedded into paraffin, sliced into 5 μm sections, and transferred onto glass slides. The slides were stained with hematoxylin-eosin reagent (Sigma-Aldrich, St. Louis, MO), and a histological examination was performed, with a focus on crypt architecture, inflammatory cell infiltration, goblet cell depletion, and muscle tissue thickening (17 (link)).