Af1700

Manufactured by Cell Signaling Technology

The AF1700 is a lab equipment product manufactured by Cell Signaling Technology. It is a general-purpose tool designed for use in various laboratory applications. The core function of the AF1700 is to facilitate specific tasks or procedures within the research environment.

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Lab products found in correlation

Af2400, by Abcam (1 mentions) Sc 5279, by R&D Systems (1 mentions) Af2085, by R&D Systems (1 mentions) Af2864, by Thermo Fisher Scientific (1 mentions) Ab8978, by Abcam (1 mentions) Mab1924, by R&D Systems (1 mentions) Lsm 710, by Zeiss (1 mentions)

2 protocols using af1700

Immunofluorescence Analysis of Pluripotency and Lineage Markers

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OCT4: Santa Cruz Biotechnology (sc-5279), 1:200

BRA: R&D Systems (MAB20851-100), 1:500

BRA: R&D Systems (AF2085), 1:300

GATA6: R&D Systems (AF1700), 1:200

GATA6: Cell Signaling (D61E4), 1:200

GATA3: Thermo Fisher Scientific (14-9966-82), 1:100

SOX17: R&D Systems (MAB1924), 1:200

HAND1: R&D Systems (AF3168-SP), 1:200

KRT7: Abcam (ab209600), 1:100

ISL1: DSHB (39.4D5), 1:200

MIXL1: Sigma Prestige antibody (HPA005662), 1:200

SUSD2: Miltenyi Biotec (130-106-401), 1:100

SSEA4: Miltenyi Biotec (130-122-958), 1:100

KLF17: Atlas Antibodies (HPA024629), 1:200

SOX17: R&D Systems (AF2864), 1:200

GATA4: Thermo Fisher Scientific (14-9980-82), 1:100

FOXA2: Novus Biologicals (AF2400), 1:200

NR2F2: Abcam [EPR18442] (ab211776), 1:100

CD24: eBioscience (A5-2H10), 1:100

Vimentin: Abcam (ab8978), 1:200

De Santis R., Rice E., Croft G., Yang M., Rosado-Olivieri E.A, & Brivanlou A.H. (2023). The emergence of human gastrulation upon in vitro attachment. Stem Cell Reports, 19(1), 41-53.

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Simultaneous Detection of GATA6 and Limb Bud Markers

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In order to clarify GATA6 localization in hindlimb bud mesenchyme, GATA6 was simultaneously detected with limb bud mesenchyme markers, such as FGF10 or DUSP6. Wild-type embryos were fixed, washed and cryosectioned as described above. Sections were simultaneously stained by anti-GATA6 (R&D AF1700 or Cell Signaling #5851, dilution 1:1,600) and anti-FGF10 (Santa Cruz, sc-7917, dilution 1:100) or anti-DUSP6 (Sigma, Clone 3G2, dilution 1:200). Sections were reacted with Alexa fluorophore-labelled secondary antibodies, and fluorescent signals were detected by Zeiss LSM 710 according to a standard procedure [13 (link)].

Hayashi S., Akiyama R., Wong J., Tahara N., Kawakami H, & Kawakami Y. (2016). Gata6-Dependent GLI3 Repressor Function is Essential in Anterior Limb Progenitor Cells for Proper Limb Development. PLoS Genetics, 12(6), e1006138.

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