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Cxlc13

Manufactured by R&D Systems
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CXLC13 is a laboratory instrument designed for the measurement and analysis of various biological samples. The core function of this product is to provide accurate and reliable data on the characteristics and properties of the samples under investigation.

Automatically generated - may contain errors

Lab products found in correlation

L glutamine, by Corning (2 mentions) 5 μm pore sized transwells, by Corning (2 mentions)

2 protocols using cxlc13

1

Chemotaxis Assay for CD4+ T Cells

Cited 1 time
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Chemotaxis assays were performed as described previously 53 (link). 5×105 enriched CD4 splenic T cells were incubated for 1 h with 1× DMEM containing 0.5% fatty acid–free BSA (EMD Biosciences), 5% antibiotics, L-glutamine (Cellgro), and Hepes. Cells were then allowed to migrate through 5-μm-pore–sized transwells (Corning) toward soluble CXCL12, CXLC13 (R&D Systems) or media alone, for 3 h at 37°C. Cells were collected, stained, and resuspended in 45 μl of staining buffer and analyzed by flow cytometry.
Weinstein J.S., Herman E.I., Lainez B., Licona-Limón P., Esplugues E., Flavell R, & Craft J. (2016). Follicular helper T cells progressively differentiate to regulate the germinal center response. Nature immunology, 17(10), 1197-1205.
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2

Chemotaxis Assay for CD4+ T Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Chemotaxis assays were performed as described previously 53 (link). 5×105 enriched CD4 splenic T cells were incubated for 1 h with 1× DMEM containing 0.5% fatty acid–free BSA (EMD Biosciences), 5% antibiotics, L-glutamine (Cellgro), and Hepes. Cells were then allowed to migrate through 5-μm-pore–sized transwells (Corning) toward soluble CXCL12, CXLC13 (R&D Systems) or media alone, for 3 h at 37°C. Cells were collected, stained, and resuspended in 45 μl of staining buffer and analyzed by flow cytometry.
Weinstein J.S., Herman E.I., Lainez B., Licona-Limón P., Esplugues E., Flavell R, & Craft J. (2016). Follicular helper T cells progressively differentiate to regulate the germinal center response. Nature immunology, 17(10), 1197-1205.
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