Proteins of cell lysates or tissue homogenates were separated by SDS–PAGE according to their molecular weight using Tris/glycine as electrophoresis buffer and were transferred onto polyvinylidene fluoride membranes (Carl Roth GmbH, Karlsruhe, Germany) using CAPS buffer. After blocking, membranes were hybridized with respective primary antibodies. Membranes were washed, incubated with respective secondary horseradish-peroxidase (HRP)-conjugated antibody and detected using ECL2 Western blotting substrate (Thermo Scientific, Waltham, MA). Antibodies used were rabbit anti-HSL, rabbit anti-phospho-HSL (S660), and rabbit anti-GAPDH (all from Cell Signaling, Danvers, MA, USA), rabbit anti-CGI-58 (Abnova, Heidelberg), mouse anti-β-Actin (Santa Cruz, Santa Cruz, CA, USA), rabbit anti-alpha smooth muscle actin (α-SMA, Pierce, Thermo Scientific), HRP-linked sheep-anti mouse antibody, (GE Healthcare Amersham, Buckinghamshire, UK), and HRP-linked rat-anti rabbit antibody (Dako, Glostrup, Denmark).
Hrp linked rat anti rabbit antibody
Manufactured by Agilent Technologies
Sourced in United Kingdom
The HRP-linked rat-anti rabbit antibody is a laboratory reagent used in immunoassay techniques. It consists of a rabbit antibody linked to horseradish peroxidase (HRP), a commonly used enzyme label. This product can be utilized in various immunodetection methods, such as Western blotting, ELISA, and immunohistochemistry, to identify and quantify target proteins.
Automatically generated - may contain errors
Lab products found in correlation
Ecl2 western blotting substrate, by Thermo Fisher Scientific (2 mentions)
Polyvinylidene fluoride membrane, by Carl Roth (2 mentions)
Mouse anti β actin, by Santa Cruz Biotechnology (2 mentions)
Rabbit anti gapdh, by Cell Signaling Technology (2 mentions)
Rabbit anti phospho hsl s660, by Cell Signaling Technology (2 mentions)
Rabbit anti cgi 58, by Abnova (2 mentions)
Hrp linked sheep anti mouse antibody, by GE Healthcare (2 mentions)
Rabbit anti hsl, by Cell Signaling Technology (2 mentions)
2 protocols using hrp linked rat anti rabbit antibody
1
Western Blot Analysis of Lipid Metabolism Proteins
2
Western Blot Analysis of Lipid Metabolism Proteins
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Proteins of cell lysates or tissue homogenates were separated by SDS–PAGE according to their molecular weight using Tris/glycine as electrophoresis buffer and were transferred onto polyvinylidene fluoride membranes (Carl Roth GmbH, Karlsruhe, Germany) using CAPS buffer. After blocking, membranes were hybridized with respective primary antibodies. Membranes were washed, incubated with respective secondary horseradish-peroxidase (HRP)-conjugated antibody and detected using ECL2 Western blotting substrate (Thermo Scientific, Waltham, MA). Antibodies used were rabbit anti-HSL, rabbit anti-phospho-HSL (S660), and rabbit anti-GAPDH (all from Cell Signaling, Danvers, MA, USA), rabbit anti-CGI-58 (Abnova, Heidelberg), mouse anti-β-Actin (Santa Cruz, Santa Cruz, CA, USA), rabbit anti-alpha smooth muscle actin (α-SMA, Pierce, Thermo Scientific), HRP-linked sheep-anti mouse antibody (GE Healthcare Amersham, Buckinghamshire, UK), and HRP-linked rat-anti rabbit antibody (Dako, Glostrup, Denmark).
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