Apoptosis was determined by flow cytometry using FITC-conjugated Annexin V/PI kit (Beyotime Biotechnology, Jiangsu, China). Briefly, cells were harvested, washed, and resuspended in binding buffer at 5 × 106 ml−1. 5 μl of FITC-conjugated Annexin V solution and 10 μl of PE-conjugated PI solution were added to 100 μl of cell suspension. After incubation with Annexin V/PI for 15 min at room temperature, samples were measured using BD FACSAria II analyzer with FL2/FL4 channels (BD, New Jersey, USA). Every sample counted 10000 events for quantification.
Fitc conjugated annexin 5 pi kit
Manufactured by Beyotime
Sourced in China
The FITC-conjugated Annexin V/PI kit is a laboratory reagent used for the detection and quantification of apoptosis in cells. It contains FITC-conjugated Annexin V, which binds to phosphatidylserine exposed on the surface of apoptotic cells, and propidium iodide (PI), which stains the DNA of cells with compromised cell membranes.
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2 protocols using fitc conjugated annexin 5 pi kit
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Annexin V and PI Apoptosis Assay
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Annexin V/PI Apoptosis Assay
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Apoptosis was determined by flow cytometry using a FITC-conjugated annexin V/PI kit (Beyotime Biotechnology, Jiangsu, China). Briefly, cells were harvested, washed, and resuspended in binding buffer provided in the kit. After 15 min incubation of annexin V/PI at room temperature, samples were measured using a BD FACSAria II analyzer with FL2/FL4 channels (BD, New Jersey, USA).
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