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Ab118587

Manufactured by Abcam
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Ab118587 is a laboratory reagent used for scientific research purposes. It is a specific antibody that can be used to detect and quantify a target protein or antigen in various experimental applications. The core function of this product is to serve as a tool for researchers to investigate biological systems and cellular processes.

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Lab products found in correlation

Ab28373, by Abcam (1 mentions) Ab175474, by Abcam (1 mentions) Ab150107, by Abcam (1 mentions) Abn78, by Abcam (1 mentions) Ab150073, by Abcam (1 mentions) Ab65783, by Abcam (1 mentions) Sk 4100, by Vector Laboratories (1 mentions) Pk 6100, by Vector Laboratories (1 mentions)

2 protocols using ab118587

1

Multimodal Neuronal Receptor Labeling

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Sections were triple-labeled with rabbit anti-NeuN (Millipore ABN78, 1/500), mouse anti-NMDAR2B (Abcam ab28373, 1/500), and goat anti-NMDAR2A (abcam ab118587, 1/500). Secondary antibodies were anti-rabbit-alexa-488, anti-goat-alexa-568, and anti-mouse-alexa-647 (Abcam ab150073, ab175474, and ab150107, respectively, 1/1000).
Bacq A., Astori S., Gebara E., Tang W., Silva B.A., Sanchez-Mut J., Grosse J., Guillot de Suduiraut I., Zanoletti O., Maclachlan C., Knott G.W., Gräff J, & Sandi C. (2018). Amygdala GluN2B-NMDAR dysfunction is critical in abnormal aggression of neurodevelopmental origin induced by St8sia2 deficiency. Molecular Psychiatry, 25(9), 2144-2161.
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2

Quantitative Immunohistochemistry for GRIN2A/B

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The same animals from in situ hybridization were used for immunohistochemistry. Fixed sections were blocked using BLOXALL (catalog # SP-6000, Vector Laboratories), rinsed, and incubated in normal blocking serum. Sections were then incubated in 1:500 primary antibody (anti-GRIN2A, catalog # Ab118587; anti-GRIN2B, catalog # Ab65783, Abcam) overnight at 4°C. They were then incubated with appropriate secondary antibodies followed by avidin-biotin complex reagent (catalog # PK-6100, Vector Laboratories). Finally, they were washed and incubated in 3,3′-diaminobenzidine (DAB) peroxidase substrate (catalog # SK-4100, Vector Laboratories) and coverslipped. They were quantified the same way as in the in situ hybridization analysis.
Audet J.N., Kayello L., Ducatez S., Perillo S., Cauchard L., Howard J.T., O’Connell L.A., Jarvis E.D, & Lefebvre L. (2018). Divergence in problem-solving skills is associated with differential expression of glutamate receptors in wild finches. Science Advances, 4(3), eaao6369.
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