Plan fluor 10 objective

Manufactured by Nikon

The Nikon Plan Fluor 10× objective is a high-quality optical lens designed for use in a variety of laboratory instruments. It provides a 10× magnification and is optimized for fluorescence microscopy applications. The objective offers a numerical aperture of 0.30 and a working distance of 16.0 mm, ensuring effective light gathering and image resolution.

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Lab products found in correlation

Plan apo 60, by Nikon (1 mentions) 24 well culture plate, by Greiner (1 mentions) Te2000 e microscope, by Nikon (1 mentions) Orca er, by Hamamatsu Photonics (1 mentions) Eclipse ti e microscope, by Nikon (1 mentions)

Close spelling variants of this product

CFI plan fluor 10 × /0.30 N.A. objective lens Plan Fluor 10×0.30 NA objective CFI Super Plan Fluor ELWD ADM 10× objective ×10 CFI Plan Fluor objective Plan Fluor 10 × 0.30 objective lens Plan Fluor 10× NA 0.30 phase contrast objective Plan Fluor × 10/0.3 NA objective Plan Fluor objective Plan Fluor 10× Plan Fluor

4 protocols using plan fluor 10 objective

Mitochondrial Imaging in Micropatterns

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Micropatterns were incubated in extracellular imaging buffer (130 mM sodium chloride, 5 mM potassium chloride, 1.5 mM calcium chloride, 1 mM magnesium chloride, 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), 1 mg/mL BSA, and 5 mM glucose, with the pH adjusted to 7.4) with 10 nM tetramethylrhodamine methyl ester (TMRM, Life Technologies) for 45 min, and imaged in the same dye-containing buffer using a Nikon Eclipse Ti inverted microscope, using a Nikon Plan Fluor 10× objective with a numerical aperture (NA) of 0.30 (for unconfined micropatterns) or a Nikon Plan Apo 20× objective with 0.75 NA (for confined micropatterns). A Nikon C2 confocal microscope (Nikon Plan Apo 60× oil immersion objective, 1.40 NA) was used for confocal imaging.

Begum H.M., Mariano C., Zhou H, & Shen K. (2021). E-Cadherin Regulates Mitochondrial Membrane Potential in Cancer Cells. Cancers, 13(20), 5054.

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Automated Cell Migration Assay on Hydrogels

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Cells were seeded in a 24 well culture plate (Greiner) at a density of 1.5 × 10⁴ cells/well on hydrogels or polystyrene in full DMEM/F12 media and incubated for 5 days at 37 °C, 5% CO₂ in automated cell culture and the analysis system Cell-IQ^® (CM Technologies Oy, Tampere, Finland). Cells were visualized by phase contrast using Nikon Plan Fluor 10× objective (NA 0.30). Each visual field was visualized for 4 h. Cell migration activity was measured using the Cell-IQ Analyzer software.

Belousov A., Patlay A., Silant’ev V., Kovalev V.V, & Kumeiko V. (2022). Preparation of Hydrogels Based on Modified Pectins by Tuning Their Properties for Anti-Glioma Therapy. International Journal of Molecular Sciences, 24(1), 630.

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Cell Morphology Analysis with siRNA

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The analysis of cell morphology was carried out as previously described [13 (link)]. Briefly, a 96-well plate was coated with 40 µl of a 7.5 mg/ml Matrigel solution. Around 5 × 10⁵ cells transfected with siRNA oligonucleotides 48 h before the assay were mixed with 7 mg/ml Matrigel solution and transferred into one of the wells in the Matrigel-coated plate. The plate was incubated at 37°C. After 2 h, 100 µl of appropriated medium without FCS was added to each well. Cells were incubated at 37°C for 24 h. Images were acquired using a Nikon TE2000-E microscope with a Plan Fluor 10× objective (Nikon) and a Hamamatsu Orca-ER digital camera.

Haga R.B., Garg R., Collu F., Borda D'Agua B., Menéndez S.T., Colomba A., Fraternali F, & Ridley A.J. (2019). RhoBTB1 interacts with ROCKs and inhibits invasion. Biochemical Journal, 476(17), 2499-2514.

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2D Scratch Assay for Cell Migration

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The 2D scratch assay was performed according to previous report.^{31 (link)} For measuring cell migration rate, a scratch assay will be used where cells will be cultured in a 6 well plate to form a confluent monolayer. A p200 pipet tip will be used to scrape the cell monolayer in a straight line to create an empty gap. Then the cells will be allowed for migration into the gap and imaged to track their migration rates. The cells were imaged on an inverted Nikon Eclipse Ti-E microscope using bright field microscopy. A Nikon Plan Fluor 10 × objective (Numerical aperture: 0.30, working distance: 16.0 mm) and a 12 V/100 W halogen lamp as light source was used. The output power of the light source was kept constant for all the imaging experiments and the exposure time of 30 ms was used to provide optimal contrast and brightness. Images were then recorded by a sCOMS camera (Dhyana 400BSI, Tucsen).

Wu Y., Ali M.R., Dong B., Han T., Chen K., Chen J., Tang Y., Fang N., Wang F, & El-Sayed M.A. (2018). Gold Nanorod-Photothermal Therapy Alters Cell Junctions and Actin Network in Inhibiting Cancer Cell Collective Migration. ACS nano, 12(9), 9279-9290.

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