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Pap2m1 t156

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PAP2M1-T156 is a laboratory equipment product from Cell Signaling Technology. It is a recombinant protein that can be used for various research applications.

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Lab products found in correlation

Odyssey clx, by LI COR (2 mentions) Sars cov 2 n, by Sino Biological (2 mentions) Ap2m1, by Abcam (2 mentions) Vinculin, by Santa Cruz Biotechnology (2 mentions) Pstat1 s727, by Cell Signaling Technology (2 mentions) β actin, by Merck Group (2 mentions) Protease and phosphatase inhibitor, by Thermo Fisher Scientific (2 mentions) Pstat1 y701, by Cell Signaling Technology (2 mentions) Ifit1, by Cell Signaling Technology (2 mentions) Irdye 680 800, by LI COR (2 mentions) Stat1, by Cell Signaling Technology (2 mentions) Pikkα βs176 180, by Cell Signaling Technology (1 mentions) Nf kb p65, by Cell Signaling Technology (1 mentions) Pnfκb p65 s536, by Cell Signaling Technology (1 mentions) Iκκ β, by Cell Signaling Technology (1 mentions) Nf κb p65, by Cell Signaling Technology (1 mentions)

2 protocols using pap2m1 t156

1

Western Blot Analysis of SARS-CoV-2 Infection

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Human cell lines were grown to 70% confluence and lysed in RIPA (10% glycerol, 50mM Tris-HCl pH 7.4, 150mM NaCl, 2mM EDTA, 0.1% SDS, 1% NP40, 0.2% sodium deoxycholate) containing protease and phosphatase inhibitors (Thermo Scientific). Proteins were separated by SDS-PAGE, transferred to a nitrocellulose membrane, blocked in 5% milk, and incubated with primary antibodies overnight at 4°C. Washed membranes were incubated for 45 min at room temperature in secondary antibody solution (LI-COR IRDye 680, 800; 1:10,000 in 5% milk), imaged on an Odyssey® CLx, and analyzed using Image Studio Software. Antibodies were used at the following dilutions: ACE2 (R&D Systems #AF933, 1:200), β-actin (Sigma #A5316, 1:5000), Vinculin (Santa Cruz #sc-73614, 1:2000), AAK1 (Bethyl #A302–146A, 1:1000), AP2M1 (Abcam #ab75995, 1:1000), pAP2M1-T156 (Cell Signaling #3843, 1:1000), SARS-CoV-2-N (Sino Biological #40588-T62, 1:500), pSTAT1-Y701 (Cell Signaling #9167, 1:1000), pSTAT1-S727 (Cell Signaling #8826, 1:1000), STAT1 (Cell Signaling #14994, 1:1000), MX1 (Cell Signaling #37849, 1:1000), IFIT1 (Cell Signaling #14769, 1:1000), pIKKα/β-S176/180 (Cell Signaling #2697, 1:1000), IKKα (Cell Signaling #11930, 1:1000), IKKβ (Cell Signaling #8943, 1:1000), pNFKB p65-S536 (Cell Signaling #3033, 1:1000), NFKB p65 (Cell Signaling #8242, 1:1000),
Puray-Chavez M., LaPak K.M., Schrank T.P., Elliott J.L., Bhatt D.P., Agajanian M.J., Jasuja R., Lawson D.Q., Davis K., Rothlauf P.W., Jo H., Lee N., Tenneti K., Eschbach J.E., Mugisha C.S., Vuong H.R., Bailey A.L., Hayes D.N., Whelan S.P., Horani A., Brody S.L., Goldfarb D., Major M.B, & Kutluay S.B. (2021). Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell. bioRxiv.
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2

SARS-CoV-2 Infection Signaling Pathway Analysis

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Human cell lines were grown to 70% confluence and lysed in RIPA (10% glycerol, 50mM Tris-HCl pH 7.4, 150mM NaCl, 2mM EDTA, 0.1% SDS, 1% NP40, 0.2% sodium deoxycholate) containing protease and phosphatase inhibitors (Thermo Scientific). Proteins were separated by SDS-PAGE, transferred to a nitrocellulose membrane, blocked in 5% milk, and incubated with primary antibodies overnight at 4°C. Washed membranes were incubated for 45 min at room temperature in secondary antibody solution (LI-COR IRDye 680, 800; 1:10,000 in 5% milk), imaged on an Odyssey® CLx, and analyzed using Image Studio Software. Antibodies were used at the following dilutions: ACE2 (R&D Systems #AF933, 1:200), β-actin (Sigma #A5316, 1:5000), Vinculin (Santa Cruz #sc-73614, 1:2000), AAK1 (Bethyl #A302-146A, 1:1000), AP2M1 (Abcam #ab75995, 1:1000), pAP2M1-T156 (Cell Signaling #3843, 1:1000), SARS-CoV-2-N (Sino Biological #40588-T62, 1:500), pSTAT1-Y701 (Cell Signaling #9167, 1:1000), pSTAT1-S727 (Cell Signaling #8826, 1:1000), STAT1 (Cell Signaling #14994, 1:1000), MX1 (Cell Signaling #37849, 1:1000), IFIT1 (Cell Signaling #14769, 1:1000), pIΚΚα/β-S176/180 (Cell Signaling #2697, 1:1000), IΚΚα (Cell Signaling #11930, 1:1000), IΚΚβ (Cell Signaling #8943, 1:1000), pNFΚB p65-S536 (Cell Signaling #3033, 1:1000), NFΚB p65 (Cell Signaling #8242, 1:1000),
Puray-Chavez M., LaPak K.M., Schrank T.P., Elliott J.L., Bhatt D.P., Agajanian M.J., Jasuja R., Lawson D.Q., Davis K., Rothlauf P.W., Liu Z., Jo H., Lee N., Tenneti K., Eschbach J.E., Shema Mugisha C., Cousins E.M., Cloer E.W., Vuong H.R., VanBlargan L.A., Bailey A.L., Gilchuk P., Crowe JE J.r., Diamond M.S., Hayes D.N., Whelan S.P., Horani A., Brody S.L., Goldfarb D., Major M.B, & Kutluay S.B. (2021). Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell. Cell Reports, 36(2), 109364.
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