Icap 7000 icp oes

All radio-TLC analyses were performed on silica gel 60 F254 plates (Merck) with 5% (w/v) NH 4 OAc in H 2 O-MeOH (1:1) as eluent, unless otherwise noted ( 52 Mn-surface was analyzed on cellulose). Quantification of radio traces was achieved on a MiniGita Star using a Beta Detector GMC probe (both Raytest), or on a Cyclone Plus Storage Phosphor System (Perkin-Elmer).
Radioactivity was measured using a Veenstra Instruments dose calibrator VDC-505. Metal content of radioactive preparations was quantified with an ICAP 7000 ICP-OES (Thermo Scientific).
Liposome sizes were measured by dynamic light scattering (DLS) on a ZetaPALS (Brookhaven).
Liposomes were sized by pressure extrusion using a 10 mL LIPEX thermobarrel pressure extruder (20 bar nitrogen pressure) [7] . Lipid concentrations were determined by an iCAPQ ICP-MS (Thermo Scientific).
An isotonic TRIS-saline buffer (ISO-TRIS) containing tris(hydroxymethyl) aminomethane (TRIS, 10 mM) and NaCl (150 mM) was prepared and adjusted to pH 7.8 with HCl/NaOH (osmolality: 280 mOsm/kg). An isotonic HEPES-saline buffer (ISO-HEPES) containing 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 10 mM) and NaCl (150 mM) was prepared by mixing molar equal amounts of sodium HEPES salt and free acid HEPES, for a final pH of 7.4-7.5 and osmolality of 292 mOsm/kg. For EDTA challenge, a 1.0 mM EDTA solution in ISO-HEPES was used (pH 7.4).