Anti cd4 pe clone a161a1

Manufactured by BioLegend

Sourced in Canada

Anti-CD4-PE (clone A161A1) is a fluorescently labeled antibody that binds to the CD4 surface antigen. CD4 is a glycoprotein found on the surface of T helper cells, monocytes, macrophages, and dendritic cells. This antibody can be used for the identification and enumeration of CD4-positive cells in flow cytometry applications.

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Lab products found in correlation

Clone okt3, by Thermo Fisher Scientific (1 mentions) Efluor 450, by Thermo Fisher Scientific (1 mentions) Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (1 mentions) Live dead fixable yellow, by Thermo Fisher Scientific (1 mentions) Anti cd8 apc clone sk1, by BioLegend (1 mentions) Facsaria 2, by BD (1 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions) Anti cd137 apc clone 4b4 1, by BD (1 mentions) Human serum, by Valley Biomedical (1 mentions)

Spelling variants of this product

Anti-CD4 (192 citations) CD4-PE (86 citations) Anti-CD4-PE (39 citations) Anti-CD4 (clone A161A1, (2 citations) Anti-CD4-PE (A161A1, (2 citations) CD4 (clone A161A1; (2 citations)

2 protocols using anti cd4 pe clone a161a1

Flow Cytometric Analysis of CD8+ T Cell Proliferation

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Flat-bottom 96 well plates were prepared with or without anti-CD3 coating at 1 μg/mL (clone OKT3, catalog 14-0037-82, eBioscience). hCTLs were stained with 10 μM cell proliferation dye eFluor 450 (65-0842-85, Thermo Fisher) for 10 minutes in the dark at 37°C, followed by quenching with FBS-containing media for 5 minutes. Subsequently, hCTLs were washed 3× with FBS-containing media, and 20,000 hCTLs were seeded per well. After 5 days, cells were washed 2× in ice-cold D-PBS (Thermo Fisher) prior to staining with an anti–CD8-APC (clone SK1, catalog 344722, BioLegend) or anti-CD4-PE (clone A161A1, catalog 357404, BioLegend) and Live/Dead fixable yellow (catalog L34959, Thermo Fisher). hCTLs were washed in PBS, 1% FCS prior to analysis on a LSR Fortessa flow cytometer. Samples were gated on live CD8⁺ cells; results were analyzed using FlowJo 10 software.

Randzavola L.O., Strege K., Juzans M., Asano Y., Stinchcombe J.C., Gawden-Bone C.M., Seaman M.N., Kuijpers T.W, & Griffiths G.M. (2019). Loss of ARPC1B impairs cytotoxic T lymphocyte maintenance and cytolytic activity. The Journal of Clinical Investigation, 129(12), 5600-5614.

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Enrichment of ZIKV-Reactive T Cells

Cited 1 time

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We modified AIM-based sorting (6 , 8 (link), 17 –19 ) to enrich ZIKV-reactive cells. Thawed PBMC were cultured at 2–4 × 10⁶/well in 24-well plates in 2 ml/well T-cell medium (TCM, RPMI 1640 with 25mM HEPES, 1% penicillin-streptomycin, 2 mM L-glutamine, 5% FCS (ThermoFisher), and 5% human serum (Valley Biomedical, Winchester, VA). Mock or ZIKV antigens were added at 1:60 dilution for 18 hours in humidified, 37°C, 5% CO₂ conditions. Cells were stained with a cocktail of anti-CD3-ECD (clone UCHT1, Beckman Coulter, Brea, CA), anti-CD4-PE (clone A161A1, Biolegend), anti-CD8-FITC (clone MHCD0801–4, ThermoFisher) and anti-CD137-APC (clone 4B4–1, BD, San Jose, CA) and 7-AAD viability stain (BD). Live CD3⁺CD8⁻CD4⁺CD137^high cells were sorted (FacsAria II, BD). The threshold for CD137 positivity was set using PBMC from separate persons seropositive for HSV-1 and stimulation with UV-treated HSV-1 antigen as described (6 ). CD137^high cells were expanded polyclonally once with phytohemagglutinin (PHA) as mitogen, and further expanded with anti-CD3 mAb as mitogen (19 ).

Campbell V.L., Nguyen L., Snoey E., McClurkan C.L., Laing K.J., Dong L., Sette A., Lindestam Arlehamn C.S., Altmann D.M., Boyton R.J., Roby J.A., Gale M J.r., Stone M., Busch M.P., Norris P.J, & Koelle D.M. (2020). Proteome-wide Zika virus CD4 T cell epitope and HLA restriction determination. ImmunoHorizons, 4(8), 444-453.

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