For Western blotting, cells were lysed using RIPA buffer. Equal amounts of protein (30–50 μg) were loaded and separated by 7.5% SDS-PAGE. Membranes were incubated overnight at 4 °C with the appropriate primary antibody followed by incubation with horseradish peroxidase-conjugated goat anti-rabbit IgG (Dako). Blots were visualized by an ECL system (Pierce). ICAM-1, VCAM-1, PECAM-1and phospho Smad 1/5 antibodies were purchased from Cell Signaling, BMPR-Ia antibody was from Abcam, and BMPR-II antibody from BD Biosciences.
Bmpr 2 antibody
Manufactured by BD
The BMPR-II antibody is a laboratory tool used to detect the presence and expression levels of the Bone Morphogenetic Protein Receptor Type II (BMPR-II) protein. BMPR-II is a transmembrane serine/threonine kinase receptor that plays a crucial role in various cellular processes. This antibody can be utilized in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to analyze BMPR-II protein expression and distribution in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Pecam 1, by Cell Signaling Technology (1 mentions)
Icam 1, by Cell Signaling Technology (1 mentions)
Vcam 1, by Cell Signaling Technology (1 mentions)
Phospho smad1 5, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase conjugated goat anti rabbit igg, by Agilent Technologies (1 mentions)
Pluronic f 127, by Thermo Fisher Scientific (1 mentions)
Trpc4 antibody, by Alomone (1 mentions)
2 protocols using bmpr 2 antibody
1
Protein Expression Analysis by Western Blotting
2
Investigating BMP4 Signaling Pathways
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Unless otherwise noted, all reagents were obtained from Sigma-Aldrich. All antibodies were obtained as follow: TRPC1, 6 antibodies (abcam), TRPC4 antibody (Alomone Laboratories), phospho-Smad1/5/8, phospho-ERK1/2, phospho-p38MAPK, ERK1/2, Smad5 and p38MAPK antibodies (Cell Signaling Technology), BMPRIa antibody (Proteintech Group), BMPRIb antibody (Invitrogen), ALK2 antibody (Novus), BMPRII antibody (BD), ActR2a and ActR2b antibodies (Abbiotec), α-actin antibody (Sigma-Aldrich).
Stock solutions of rhBMP4 at 50 µg/ml were made in 4 mM HCl containing 0.1% BSA. Stock solutions 30 mM of CPA and 5 mM nifedipine were made in dimethyl sulfoxide (DMSO) and 200 mM of MnCl2 was dissolvd in deionized water. Fura-2 AM was prepared as a 2.5 mM stock solution in DMSO containing 20% pluronic F-127 (Invitrogen).
Stock solutions of rhBMP4 at 50 µg/ml were made in 4 mM HCl containing 0.1% BSA. Stock solutions 30 mM of CPA and 5 mM nifedipine were made in dimethyl sulfoxide (DMSO) and 200 mM of MnCl2 was dissolvd in deionized water. Fura-2 AM was prepared as a 2.5 mM stock solution in DMSO containing 20% pluronic F-127 (Invitrogen).
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