Bs1813r

Manufactured by Bioss Antibodies

Sourced in China

Bs1813R is a laboratory instrument designed for the purification and analysis of proteins, antibodies, and other biological molecules. It utilizes column chromatography techniques to separate and isolate target compounds from complex mixtures. The core function of Bs1813R is to provide a reliable and efficient means of purifying and characterizing biomolecules for further research and development applications.

Automatically generated - may contain errors

Lab products found in correlation

Ripa lysis buffer, by Beyotime (1 mentions) Ab19845, by Abcam (1 mentions) Ab13979, by Abcam (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Imagequant las 4000 mini instrument, by GE Healthcare (1 mentions) Ab9485, by Abcam (1 mentions) Las 4000, by Cytiva (1 mentions) Ckx53, by Olympus (1 mentions) Ab34100, by Abcam (1 mentions) Bs 0549r, by Bioss Antibodies (1 mentions) Rt 6100, by Rayto (1 mentions) Bs 0086r, by Bioss Antibodies (1 mentions) Bs 10423r, by Bioss Antibodies (1 mentions) Bs 1313r, by Bioss Antibodies (1 mentions) Budesonide, by AstraZeneca (1 mentions)

2 protocols using bs1813r

Protein Extraction and Western Blot Analysis

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The cells were extracted into RIPA lysis buffer (Beyotime, Beijing, China) containing a protease inhibitor cocktail (Sigma-Aldrich, Saint Louis, MO, USA), and the lysates were centrifuged at 12,000 rpm (4°C for 15 min). Protein concentration was quantified using a BCA protein assay kit (Thermo Scientific, Waltham, MA, USA). The following primary antibodies were used: rabbit anti-FABP4 (1:500; ab13979; Abcam, Cambridge, MA, USA), rabbit anti-FABP5 (1:1,000; bs-2370R; Bioss, Bejing, China), rabbit anti-PPAR-γ (1:1,000; 2443S; Cell Signaling Technology, Danvers, MA, USA) and rabbit anti-CADM2 (1:1,000; bs-8246R; Bioss, Bejing, China), rabbit anti-CADM3 (1:200; 343894; USBiological, Swampscott, MA, USA), rabbit anti-CADM4 (1:1,000; bs-5997R; Bioss, Bejing, China), rabbit anti-HDAC1 (1:1,000; ab19845; Abcam, Cambridge, MA, USA), rabbit anti-HDAC2 (1:1,000; bs1813R; Bioss, Bejing, China), rabbit anti-HDAC6 (1:1,000; bs-2811R; Bioss, Bejing, China), rabbit anti-HDAC8 (1:1,000; bsm-52088R; Bioss, Bejing, China), rabbit anti-GAPDH (1:1,000; ab9485; Abcam, Cambridge, MA, USA) were used as a control. Signal detection was performed using an ImageQuant LAS 4000 mini instrument (GE Healthcare, Piscataway, NJ, USA).

Chen Q.Y., Huang X.B., Zhao Y.J., Wang H.G., Wang J.B., Liu L.C., Wang L.Q., Zhong Q., Xie J.W., Lin J.X., Lu J., Cao L.L., Lin M., Tu R.H., Zheng C.H., Li P, & Huang C.M. (2022). The peroxisome proliferator-activated receptor agonist rosiglitazone specifically represses tumour metastatic potential in chromatin inaccessibility-mediated FABP4-deficient gastric cancer. Theranostics, 12(4), 1904-1920.

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Collagen and HDAC2 Regulation in Angiogenesis

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Erythromycin enteric-coated tablets (H42021990, Yichang Humanwell Pharmaceutical Co., LTD.); Vorinostat Capsules (180509, Beijing Hengrui Kangda Medical Science and Technology Development Co., Ltd.); Budesonide (AstraZeneca 8339000); Rabbit Anti-Collagen III Polyclonal Antibody (bs-10423R, Bioss); Rabbit Anti-Collagen I Polyclonal Antibody (bs-0549R, Bioss); Rabbit Anti-HDAC2 Polyclonal Antibody (bs-1813R, Bioss); Rabbit VEGF ELISA kit (MM-021001); Rabbit TGF-β1 ELISA kit (MM-3684001); Rabbit Polyclonal Anti-VEGF (bs-1313R, Bioss, 1/500–1/2000); Rabbit Polyclonal Anti-TGFβ1 (bs-0086R, Bioss, 1/500–1/2000); Rabbit monoclonal Anti-IL-8 (ab34100, abcam, 1/1000); Rabbit Polyclonal Anti-HDAC2 (OmnimAbs, OM105905, 1/500–1/2000); fluorescence microscope (CKX53, OLYMPUS); Microplate Reader (RT-6100, Rayto); Protein vertical electrophoresis instrument (DYY-6C, Beijing 61 instrument factory); Ultra High Sensitivity Chemiluminescence Imaging System (Chemi DocTM XRS+, Bio-Rad Shanhhai Laboratories).

Huang Z., Wei P., Gan L., Zeng T., Qin C, & Liu G. (2020). Role of Erythromycin-Regulated Histone Deacetylase-2 in Benign Tracheal Stenosis. Canadian Respiratory Journal, 2020, 4213807.

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