Immersol medium

Manufactured by Zeiss

Sourced in Germany

Zeiss immersol medium is an immersion oil designed for use with microscopes. It is a clear, colorless liquid that is used to improve the optical performance of microscope objectives by minimizing refractive index differences between the specimen, coverslip, and objective lens.

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Lab products found in correlation

Streptavidin apc cy7, by BD (1 mentions) Cd11b apc m1 70, by Thermo Fisher Scientific (1 mentions) F4 80 pe bm8, by Thermo Fisher Scientific (1 mentions) C kit apc 2b8, by BD (1 mentions) Cd45 fitc hi30, by BD (1 mentions)

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Immersol

2 protocols using immersol medium

Comprehensive Hematopoietic Lineage Staining

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Monoclonal antibodies for lineage staining used were Gr1-PE (clone-RB6-8CS), B220-PE (RA3-6B2), Sca-1-PE (D7), ckit-APC (2B8) and CD45-FITC (HI30) from BD Biosciences, CD11b-APC (M 1/70) and F4/80-PE (BM8) from eBioscience. Antibodies used for the lineage cocktail were PerCP Cy5.5 labelled CD4 (GK1.5) and Ter119 (Ter-119) from Biolegend, B220 (RA3-6B2) and CD3 (145-2C11) from BD Biosciences and Gr-1 (RB6-8C5C), CD8 (53-6.7), IL7R (A7R34), CD11b (M1/70) from eBioscience. Antibodies used for SLAM HSC staining were CD48-APC (HM48-1), CD150-PECy7 (TC-15-12F12.2) from Biolegend and CD244-PE (2B4) from eBioscience. Antibodies used for progenitor staining were CD16/32-PE (93), CD34-biotin (RAM34), Sca1-PECy7 (D7) from eBioscience, ckit-APC (2B8) and Streptavidin-APC-Cy7 from BD Biosciences. Cytospin preparations were stained with Wright-Giemsa stain. The morphology of bone marrow was assessed with an Olympus BX51 (Olympus, Tokyo, Japan) microscope and a 40x/0.75 numerical aperture objective, or a 100x/1.3 numerical aperture objective with Zeiss immersol medium (Zeiss, Jena, Germany). OlympusXC50 (Olympus) and analySIS software (Soft Imaging System, Stuttgart, Germany) were used to capture images.

Chaturvedi A., Araujo Cruz M.M., Jyotsana N., Sharma A., Goparaju R., Schwarzer A., Görlich K., Schottmann R., Struys E.A., Jansen E.E., Rohde C., Müller-Tidow C., Geffers R., Göhring G., Ganser A., Thol F, & Heuser M. (2016). Enantiomer-specific and paracrine leukemogenicity of mutant IDH metabolite 2-hydroxyglutarate. Leukemia, 30(8), 1708-1715.

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Giemsa Staining for Bone Marrow Smears

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The Giemsa staining method used for BM smears is described in the Supplementary Material. The morphology of bone marrow was assessed with an Olympus BX51 (Olympus, Tokyo, Japan) microscope and a 40×/0.75 numerical aperture objective or a 100×/1.3 numerical aperture objective with Zeiss Immersol Medium (Zeiss, Jena, Germany). OlympusXC50 (Olympus) and analySIS software (Soft Imaging System, Stuttgart, Germany) were used to capture images.

Braun T., Pruene A., Darguzyte M., vom Stein A.F., Nguyen P.H., Wagner D.L., Kath J., Roig-Merino A., Heuser M., Riehm L.L., Schneider A., Awerkiew S., Talbot S.R., Bleich A., Figueiredo C., Bornhäuser M, & Stripecke R. (2023). Non-viral TRAC-knocked-in CD19KICAR-T and gp350KICAR-T cells tested against Burkitt lymphomas with type 1 or 2 EBV infection: In vivo cellular dynamics and potency. Frontiers in Immunology, 14, 1086433.

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