Nis element advanced research software 4

Data were analyzed using Prism 10.0 (GraphPad Software Inc., San Diego, CA, USA). All data are presented as means ± SD from three to five independent experiments performed in duplicates unless otherwise indicated. The graphs were generated using the same software. Sigmoidal dose-response (variable slope) parameters were used for analyzing the data to calculate EC₅₀ and E_max values. To determine the level of significance, data were analyzed using one-way ANOVA with Bonferroni’s multiple comparisons test and multiple t-tests with Welch correction were used for pairwise comparisons between two groups (Prism 10.0). A p-value of less than or equal to 0.05 was considered to be significant and exact p-values are disclosed in a Source Data file (Supplementary Data 3). The NIS Element Advanced Research software 4.0 was used for microscopy image acquisition and analysis.

The data were analyzed using Prism 8.0 (GraphPad Software Inc., San Diego, CA, USA). Differences between means were tested for significance by 2-way ANOVA or repeated measures 2-way ANOVA and Dunnet’s multiple comparisons test. The NIS Element Advanced Research software 4.0 was used for microscopy image acquisition and analysis.