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Amaxa cell line nucleofactor kit c

Manufactured by Lonza
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Sourced in Germany

The Amaxa Cell Line Nucleofactor Kit C is a laboratory equipment product designed for the transfection of cell lines. It facilitates the introduction of nucleic acids, such as plasmids or siRNA, into a variety of mammalian cell types.

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Lab products found in correlation

On targetplus smartpool, by Thermo Fisher Scientific (1 mentions) Dual luciferase reporter assay, by Promega (1 mentions)

Spelling variants of this product

Amaxa Nucleofactor (13 citations) Nucleofactor Kit (6 citations) Amaxa Nucleofactor kit (5 citations) Amaxa kits (2 citations)

2 protocols using amaxa cell line nucleofactor kit c

1

Silencing CD36 and iPLA2β in U937 Cells

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To silence the expression of CD36 and iPLA2β in U937 cells, Amaxa Cell Line Nucleofactor Kit C and Nucleofactor II Device (both from Lonza Cologne, Cologne, Germany) were used. Cells were transfected with ON-TARGETplus SMARTpool siRNA targeting human CD36 and iPLA2β (CD36 and PLA2G6), as well as with Non-targeting Control Pool (Thermo Fisher Scientific, Schwerte, Germany) at a concentration of 30 pM siRNA/1 × 106 cells.
Siebers K., Fink B., Zakrzewicz A., Agné A., Richter K., Konzok S., Hecker A., Zukunft S., Küllmar M., Klein J., McIntosh J.M., Timm T., Sewald K., Padberg W., Aggarwal N., Chamulitrat W., Santoso S., Xia W., Janciauskiene S, & Grau V. (2018). Alpha-1 Antitrypsin Inhibits ATP-Mediated Release of Interleukin-1β via CD36 and Nicotinic Acetylcholine Receptors. Frontiers in Immunology, 9, 877.
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2

CD47 Enhancer Luciferase Assay

Cited 1 time
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416b cells were transfected using Amaxa® Cell Line Nucleofactor® kit C (Lonza), U937 program, with 2 μg of RENILLA Luciferase control reporter and 4 μg of CD47 enhancer-Firefly vectors. CD47 enhancer-FIREFLY vector was generated from the previously described construct CD47-E5 (28 (link)). Luciferase activity was monitored 8 h after transfection using the Dual Luciferase Reporter Assay (Promega).
Poplineau M., Vernerey J., Platet N., N’guyen L., Hérault L., Esposito M., Saurin A.J., Guilouf C., Iwama A, & Duprez E. (2019). PLZF limits enhancer activity during hematopoietic progenitor aging. Nucleic Acids Research, 47(9), 4509-4520.
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