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Cyctochalasin d

Manufactured by Merck Group
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Cytochalasin D is a fungal metabolite that acts as an inhibitor of actin polymerization. It binds to the fast-growing end of actin filaments, preventing further addition of actin monomers and leading to the disruption of the actin cytoskeleton.

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Lab products found in correlation

Idelalisib, by Selleck Chemicals (2 mentions) Entospletinib, by Selleck Chemicals (2 mentions) Acalabrutinib, by Selleck Chemicals (2 mentions) Dyngo 4a, by Selleck Chemicals (2 mentions) Ibrutinib, by Selleck Chemicals (2 mentions) Polybead carboxylate microspheres, by Polysciences (2 mentions) Dynabeads m 280, by Thermo Fisher Scientific (2 mentions)

2 protocols using cyctochalasin d

1

Inhibitor Assays with Antibody Conjugates

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Ibrutinib, acalabrutinib, entospletinib, idelalisib and dyngo-4A (SelleckChem), and cyctochalasin D (Merck) were dissolved in dimethyl sulfoxide (DMSO). The antibodies used were goat F(ab’)2 anti-human IgM, control goat F(ab’)2 and mouse F(ab’)2 κ anti-IgM (all Cambridge Biosciences). These were used as soluble antibodies or after covalent binding to 2.8 μm epoxy-coated M-280 Dynabeads (ThermoFisher) or 3 μm carboxylate-coated latex beads (Polybead® Carboxylate Microspheres; PolySciences) using the manufacturers’ instructions.
Minton A.R., Smith L.D., Bryant D.J., Strefford J.C., Forconi F., Stevenson F.K., Tumbarello D.A., James E., Løset G.Å., Munthe L.A., Steele A.J, & Packham G. (2022). B-cell receptor dependent phagocytosis and presentation of particulate antigen by chronic lymphocytic leukemia cells. Exploration of Targeted Anti-tumor Therapy, 3(1), 37-49.
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2

Kinase Inhibitor Assay Protocol

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Ibrutinib, acalabrutinib, entospletinib, idelalisib and dyngo-4A (SelleckChem), and cyctochalasin D (Merck) were dissolved in dimethyl sulfoxide (DMSO). The antibodies used were goat F(ab’)2 anti-human IgM, control goat F(ab’)2 and mouse F(ab’)2 κ anti-IgM (all Cambridge Biosciences). These were used as soluble antibodies or after covalent binding to 2.8 μm epoxy-coated M-280 Dynabeads (ThermoFisher) or 3 μm carboxylate-coated latex beads (Polybead® Carboxylate Microspheres; PolySciences) using the manufacturers’ instructions.
Minton A.R., Smith L.D., Bryant D.J., Strefford J.C., Forconi F., Stevenson F.K., Tumbarello D.A., James E., Løset G.Å., Munthe L.A., Steele A.J, & Packham G. (2022). B-cell receptor dependent phagocytosis and presentation of particulate antigen by chronic lymphocytic leukemia cells. Exploration of Targeted Anti-tumor Therapy, 3(1), 37-49.
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