Rabbit anti pericentrin

Manufactured by BioLegend

Rabbit anti-pericentrin is a primary antibody that recognizes the pericentrin protein. Pericentrin is a large coiled-coil protein that plays a key structural role in the organization of the centrosome. The antibody can be used to detect and localize pericentrin in various cell and tissue types.

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Anti-pericentrin (2 citations)

2 protocols using rabbit anti pericentrin

Comprehensive Antibody Panel for Cell Characterization

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The following primary antibodies were used in this study: mouse anti-Snail1 (1/200; a kind gift from Antonio García de Herreros, Institut Hospital del Mar d’Investigacions Mèdiques, Barcelona, Spain); mouse anti-STRIP1 (clone 7G7; 1/1,000; Origene); rabbit anti-STRIP1 (1/5,000; Bethyl Laboratories); mouse anti-SOX2 (E-4; 1/2,000; Santa Cruz Biotechnology); rabbit anti-pericentrin (1/3,000; Biolegend); rabbit anti-FOXA2 (1/200; Abcam); goat anti-Brachyury (T; 1/500; R&D Systems); rabbit anti–N-Cadherin (1/100) and rabbit anti-RAB11 (1/200; Cell Signaling); rabbit anti–phospho-histone H3 (1/500; Millipore); from Sigma-Aldrich, rabbit anti-LAMININ (1/500) and mouse anti-VINCULIN (1/200); from Thermo Fisher Scientific, rabbit-anti-GFP (1/1,000; Life Technologies) and mouse anti-ZO-1 (1/200; Zymed); from BD Biosciences, goat anti-SOX17 (1/200), mouse anti-E-Cadherin (1/400), mouse anti-GM130 (1/1,000), and mouse anti-PAXILLIN (1/200). Rhodamine-conjugated phalloidin (1/1,000) and secondary antibodies (1/1,000; Alexa Fluor 488, 568, 594, 633, or 647) were obtained from Thermo Fisher Scientific. HRP-conjugated secondary antibodies for Western blots were from GE Healthcare and used at 1/10,000.

Bazzi H., Soroka E., Alcorn H.L, & Anderson K.V. (2017). STRIP1, a core component of STRIPAK complexes, is essential for normal mesoderm migration in the mouse embryo. Proceedings of the National Academy of Sciences of the United States of America, 114(51), E10928-E10936.

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Immunohistochemical Staining Protocols

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Primary antibodies used were: Rabbit anti-Lin28 (1:500, Cell signaling, 3978); Mouse antiLin28 (1:500, Cell Signaling, 5930); Guinea pig anti-RBPMS (1:2000, Raygene A008712); Rabbit anti-c-Fos (1:500, Cell Signaling, 2250); Rabbit anti-IGF1R ((31, 33) 1:500, Santa Cruz, sc-712), rabbit anti-AP2 (1:200, Abcam, 52222), mouse anti-AP2 (1:50, Developmental Studies Hybridoma Bank, 3B5), rabbit anti-pericentrin (1:500, Biolegend, 923701), rabbit anti-adenylate cyclase III (1:500, Thermofisher, PA5–35382) rabbit anti-phospho S6 (1:100, Cell Signaling, 4857), rabbit anti-TrkB (1:20, Thermofisher, PA5–78405), goat anti-osteopontin 1 (1:400x R&D Systems, AF1433), and goat anti-choline acetyl transferase (1:400x Millipore, AB144P). Secondary antibodies were used from Jackson ImmunoResearch or Life Technologies, raised in either goat or donkey against primary antibody’s host species, highly cross adsorbed and conjugated to fluorophores of Alexa Fluor 488, Alexa Fluor 568, or Alex Fluor 647, and used at a 1:400–500 dilution. For microruby amplification, streptavidin conjugated to Alexafluor 568 (1:1000, life Technologies, S11226) was used to amplify against the biotin tag.

Zhang Y., Williams P.R., Jacobi A., Wang C., Goel A., Hirano A.A., Brecha N.C., Kerschensteiner D, & He Z. (2019). Elevating Growth Factor Responsiveness and Axon Regeneration by Modulating Presynaptic Inputs. Neuron, 103(1), 39-51.e5.

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