After staining with propidium iodide (PI) for cell cycle analysis using a cell cycle kit, the cells were counted with a FACSCalibur flow cytometer in accordance with the manufacturer's instructions (Multisciences, China).
Facscalibur flow cytometer
Manufactured by MultiSciences Biotech
Sourced in China
The FACSCalibur flow cytometer is a laboratory instrument designed for the analysis of cells and particles. It utilizes fluidics, optics, and electronics to detect and measure various characteristics of individual cells or particles as they flow through a laser beam. The core function of the FACSCalibur is to provide quantitative data on the physical and fluorescent properties of samples.
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Lab products found in correlation
2 protocols using facscalibur flow cytometer
1
Cell Cycle Analysis with PI Staining
2
Cell Cycle and Apoptosis Analysis
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For cell cycle analysis, the cells were trypsinized and fixed with 75% ethanol. The cells were washed and resuspended in 300 µl DNA staining solution (Multiscience, China), followed by analysis using a FACSCalibur flow cytometer. Cell apoptosis was detected by using the Annexin V-FITC/Propidium Iodide (PI) Apoptosis Detection Kit (BD Biosciences, USA) according to the manufacturer's instructions. Each measurement was performed in triplicate.
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