Anti stat1 clone d1k9y

Manufactured by Cell Signaling Technology

Anti-STAT1 (clone D1K9Y) is a primary antibody produced by Cell Signaling Technology. It is designed to detect endogenous levels of STAT1 protein.

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Lab products found in correlation

Alexa fluor 594 goat anti rabbit igg h l, by Thermo Fisher Scientific (1 mentions) Anti rabbit igg h l hrp conjugate, by Promega (1 mentions) Anti β catenin, by Abcam (1 mentions) Alexa fluor 568 goat anti mouse igg h l, by Thermo Fisher Scientific (1 mentions) Anti mouse igg h l hrp conjugate, by Promega (1 mentions) Anti β actin clone ac 15, by Merck Group (1 mentions) Dynabeads protein a, by Thermo Fisher Scientific (1 mentions) Phenol chloroform isoamyl alcohol, by Merck Group (1 mentions) Rabbit igg clone da1e, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

STAT1 (305 citations) Anti-STAT1 (160 citations) Anti-STAT1 (D1K9Y) (6 citations) STAT1 (D1K9Y, (3 citations)

2 protocols using anti stat1 clone d1k9y

Immunofluorescence and Western Blot Antibodies

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Other antibodies used were as follows: mouse mAbs used were anti-K14 (Purkis et al., 1990 (link)), anti-desmoplakin (11-5F) (gifted by David Garrod, The University of Manchester, Manchester, United Kingdom), anti–β-actin (clone AC-15, number A5441, Sigma-Aldrich, St. Louis, MO), anti–β-catenin (number ab16051, Abcam, Cambridge, United Kingdom; clone 14, number 610153, BD Biosciences, San Jose, CA), and anti–keratin 17 (number NCL-L-CK17, Leica Biosystems, Richmond, IL); rabbit mAbs or polyclonal antibodies used were anti–phospho-STAT1 (Tyr701) (clone D4A7, number 7649, Cell Signaling Technology, Danvers, MA), anti-STAT1 (clone D1K9Y, number 14994, Cell Signaling Technology), and anti-phospho-Histone H3 (Ser10) Antibody (clone MC463, number 04-817, Merck Millipore, Burlington, MA); secondary antibodies used were goat anti-rabbit IgG (H+L) Alexa Fluor 594 (number A-11037, Thermo Fisher Scientific); goat anti-mouse IgG (H+L) Alexa Fluor 568 (number A-11031, Thermo Fisher Scientific); Anti-Mouse IgG (H+L), HRP Conjugate (number W4021, Promega, Madison, WI); and Anti-Rabbit IgG (H+L), HRP Conjugate (number W4011, Promega); and antibody to human kappa light chain conjugated to horseradish peroxidase was used in ELISA (kindly provided by Prof PG Sveshnikov, Russian Research Center for Molecular Diagnostics and Treatment, Moscow, Russia).

Badowski C., Tan T.S., Aliev T., Trudil D., Larina M., Argentova V., Firdaus M.J., Benny P., Woo V.S, & Lane E.B. (2022). Detrimental Effects of IFN-γ on an Epidermolysis Bullosa Simplex Cell Model and Protection by a Humanized Anti–IFN-γ Monoclonal Antibody. JID Innovations, 2(2), 100096.

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Chromatin Immunoprecipitation Protocol for STAT1 and H3K27ac

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Around 3-4 x 10⁶ cells were crosslinked with 1% PFA at room temperature for 10 minutes, quenched with 125 mM Glycine, and rinsed twice with PBS. Cells were lysed and sonicated at 4°C using a Bioruptor Sonicator (Diagenode, UCD-200TM) into 100-600 bp fragments. For STAT1 ChIP, Dynabeads Protein A (Invitrogen, 10002D) was pre-coated with 10 μg anti-STAT1 (clone D1K9Y, Cell Signaling Technology, cat#14994S) or Rabbit IgG (clone DA1E, Cell Signaling Technology, cat#3900S) antibody. For H3K27ac ChIP, Dynabeads Protein A (Invitrogen, 10002D) was pre-coated with 5 μg anti-H3K27ac (clone D5E4, Cell Signaling Technology, cat#8173S) or Rabbit IgG (clone DA1E, Cell Signaling Technology, cat#3900S) antibody. The samples were incubated with antibody pre-coated beads at 4°C overnight. ChIP samples were then washed, eluted, and reverse crosslinked by overnight incubation at 65 °C. ChIP-DNA was purified by Phenol:Chloroform:Isoamyl Alcohol (Sigma-Aldrich, 77677-100ML) and used for qPCR analysis. ChIP enrichment was calculated relative to input samples. Primers used for ChIP-qPCR are listed in Supplementary Table 3.

Cui J., Zhang C., Lee J.E., Bartholdy B.A., Yang D., Liu Y., Erler P., Galbo PM J.r., Hodge D.Q., Huangfu D., Zheng D., Ge K, & Guo W. (2023). MLL3 Loss Drives Metastasis by Promoting a Hybrid Epithelial-Mesenchymal Transition State. Nature cell biology, 25(1), 145-158.

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