Triton wr 1339 tyloxapol

Manufactured by Merck Group

Sourced in United States

Triton WR-1339 (Tyloxapol) is a non-ionic detergent commonly used in laboratory applications. It functions as a surfactant, reducing surface tension and facilitating the solubilization and dispersion of various biological samples and compounds.

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Lab products found in correlation

Simvastatin, by Merck Group (2 mentions) Chrysin, by Merck Group (1 mentions) Polysorbate 80 tween 80, by Merck Group (1 mentions) Triglyceride assay kit, by Abcam (1 mentions) Catalase assay, by Cayman Chemical (1 mentions) R carvone, by Merck Group (1 mentions) Mtt kit, by Promega (1 mentions)

Spelling variants of this product

Tyloxapol (173 citations) Triton (168 citations) Triton WR-1339 (44 citations) WR-1339 (2 citations)

6 protocols using triton wr 1339 tyloxapol

Antihyperlipidemic Effects of Natural and Synthetic Compounds

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Chrysin, simvastatin and Triton WR-1339 (Tyloxapol) (Fig. 1) were purchased from Sigma–Aldrich (St. Louis, MO, USA). simvastatin and Triton WR-1339 were dissolved in saline solution (pH 7.4) and Chrysin was dissolved in PEG (Polyetylenoglicol 20%) and saline solution (pH 7.4). The doses of the compounds used in this study are: Triton WR-1339 (400 mg/kg, 2.5 ml/kg, i.p.) based on [7] (link); Chrysin (10 mg/kg; 10 ml/kg, p.o.), based on a pilot study previously conducted by our research group in C57BL/6 mice, that demonstrated that the compound is safe in this dose; and simvastatin (10 mg/kg body W.T.) was used as the reference standard drug for evaluating the antihyperlipidemic activity, based on Sikarwar and Patil [26] (link), since it is effective in reduces plasma cholesterol by inhibiting HMG-CoA reductase activity and reduces the risk of coronary events during treatment. All other chemicals were obtained from analytical grade or from standard commercial suppliers.Fig. 1

Structure of Chrysin and Triton WR-1339.

Zarzecki M.S., Araujo S.M., Bortolotto V.C., de Paula M.T., Jesse C.R, & Prigol M. (2014). Hypolipidemic action of chrysin on Triton WR-1339-induced hyperlipidemia in female C57BL/6 mice. Toxicology Reports, 1, 200-208.

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Measuring Hepatic Triglyceride Secretion

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To assess hepatic TG secretion, 500 mg kg⁻¹ Triton WR-1339 (Tyloxapol, Sigma-Aldrich Co., St Louis, MO, USA), which blocks lipolysis of TGs in peripheral tissue, was injected into the tail veins of 6 h-fasted mice 5 days after adenovirus administration. Blood samples were taken immediately before and 30, 60 and 90 min after injection, followed by measurement of serum TG concentrations.

Uno K., Yamada T., Ishigaki Y., Imai J., Hasegawa Y., Sawada S., Kaneko K., Ono H., Asano T., Oka Y, & Katagiri H. (2015). A hepatic amino acid/mTOR/S6K-dependent signalling pathway modulates systemic lipid metabolism via neuronal signals. Nature Communications, 6, 7940.

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Carnauba Wax-Based Lipid Nanocarriers

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The carnauba wax was obtained from Pontes Indústria de Ceras Ltda., Fortaleza, CE, Brazil. Cholic acid (Inlab ® ) was obtained from Medical laboratory (Sao Luis, MA, Brazil); Cholesterol, Ethylene Dichloride P.A., Ethanol P.A., Formic aldehyde., Heptane P.A., Isopropanol P.A., and Silica gel (Vetec ® ) for the column were purchased from the Vetec Quimica Fina Ltda -Brazil. Sodium heparin (Cristalia ® ) was purchased from Cristália (Itapira, SP, Brazil), Kits for biochemical and hematological analysis were purchased from Labtest ® (Lagoa Santa, MG, Brazil). Polysorbate 80 (Tween 80)., Triton WR-1339 (tyloxapol), were purchased from the Sigma-Aldrich Co. (St. Louis, MO, USA); Simvastatin and gemfibrozil were purchased from the Merck & Co., Inc. (Whitehouse Station, NJ -USA). All other reagents and solvents used in this research were of analytical grade.

Filho A.C.V.A., Rodrigues P.A.S., Benjamin S.R., Paim R.T.T., Holanda M.O., Silva J.Y.G., Milo T.S., Vieira I.G.P., Queiroz M.G.R, & Guedes M.I.F. (2017). Hypolipidemic activity of P-methoxycinnamic diester (PCO-C) isolated from Copernicia prunífera against Triton WR-1339 and hyperlipidemic diet in mice. Environmental toxicology and pharmacology, 56.

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Liver Lipid Profiling and VLDL-TG Secretion

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Serum levels of alanine aminotransferase, aspartate aminotransferase, total cholesterol, and triglycerides were monitored by standard clinical chemistry assays on an automatic chemistry analyzer (7020; Hitachi, Tokyo, Japan). Hepatic lipids were extracted using a chloroform/methanol mix (2:1, vol/vol), as described previously,⁵⁴ (link) and total cholesterol and triglyceride levels in the liver were measured using cholesterol/cholesteryl ester assay kits (Abcam, Cambridge, United Kingdom) and triglyceride assay kits (Abcam, Cambridge, United Kingdom), respectively. The levels of free fatty acids in the liver were analyzed with gas chromatography–triple quadrupole tandem mass spectrometry. To measure Very-low-density lipoprotein (VLDL)-TG secretion rates, mice were fasted for 16 hours, pre-bled by retro-orbital bleeding, and administered intravenous injections of 10% tyloxapol (Triton WR-1339, 500 mg/kg body weight; Sigma-Aldrich, St.Louis, MO). Plasma samples were drawn serially at 0, 60, and 120 minutes after injection, and plasma TG levels were measured using triglyceride assay kits (Abcam, Cambridge, United Kingdom).

Choi Y., Song M.J., Jung W.J., Jeong H., Park S., Yang B., Lee E.C., Joo J.S., Choi D., Koo S.H., Kim E.K., Nam K.T, & Kim H.P. (2021). Liver-Specific Deletion of Mouse CTCF Leads to Hepatic Steatosis via Augmented PPARγ Signaling. Cellular and Molecular Gastroenterology and Hepatology, 12(5), 1761-1787.

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In Vivo and In Vitro VLDL Secretion

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For in vivo VLDL secretion in mice, 6- to 8-week-old C57BL/6J male mice were infected with 2.5 × 10¹¹ recombinant adenovirus particles of Ad-Scramble or Ad-shlncRHL through tail vein injection for 7 days. After 8 h fasting, mouse were injected with the lipase inhibitor tyloxapol (Triton WR-1339, 500 mg/kg i.v.; Sigma-Aldrich) and 20 mL blood was collected from the retro-orbital plexus in heparinized tubes at the indicate time point. The collected blood samples were used for TAG measurements, and the VLDL-TAG production rate was calculated from the slope of the plasma TAG versus time curve as previously described (24 (link)). For in vitro VLDL secretion experiments, after 4-h attachment, primary mouse hepatocytes isolated from 6- to 8-week-old C57BL/6J mice were transfected with ssNC or sslncRHL for 6 h. After that, those primary hepatocytes were incubated for 6 h with DMEM containing 0.4 mmol/L oleic acid/0.5% fatty acid–free BSA. Cells were then washed with PBS and incubated for a further 16 h with serum-free and phenol red–free DMEM; then the cells and medium were collected and assayed for TAG with an enzymatic TAG assay kit (Wako) and mouse VLDL ELISA kit (Jiancheng Bioengineering Research Institute, Jiangsu, China), respectively, as previously described (19 (link)).

Shen X., Zhang Y., Ji X., Li B., Wang Y., Huang Y., Zhang X., Yu J., Zou R., Qin D., Zhou H., Wang Q, & Li J.Z. (2022). Long Noncoding RNA lncRHL Regulates Hepatic VLDL Secretion by Modulating hnRNPU/BMAL1/MTTP Axis. Diabetes, 71(9), 1915-1928.

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In Vivo and In Vitro Evaluation of (R)-(−)-carvone

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(R)-(−)-carvone and tyloxapol (Triton WR 1339) were purchased from Sigma-Aldrich (USA) and fenofibrate (Trigless) was purchased from Jordan Sweden Medical and Sterilization Company (JOSWE), Jordan. Kits for glucose (code: 11503), plasma TGs (code: 11529), total cholesterol (TC) (code: 11505), high-density lipoprotein cholesterol (HDL-C) (code: 11648) and low-density lipoprotein cholesterol (LDL-C), and precipitating reagent (code: 11579) were purchased from BioSystems (S.A., Barcelona Spain). Total glutathione (GSH) (catalog number: 703002) and catalase assay (catalog number: 707002) were supplied by Cayman Chemical Co., USA. The insulin-secreting human pancreatic β-cell line 1.1E7 was obtained from the European Collection of Cell Cultures (ECACC), United Kingdom. The MTT kit was from Promega (USA). All other chemicals were of analytical grade. (R)-(−)-carvone was emulsified in 2% Tween 20 for in vivo studies. For in vitro studies, (R)-(−)-carvone was dissolved in dimethyl sulfoxide (DMSO) and diluted to prepare the various concentrations so that the final DMSO concentration was less than 0.5%. Fenofibrate and tyloxapol were dissolved in normal saline. All reagents were freshly prepared before use.

Abbas M.A., Oriquat G.A., Abbas M.M., Al-Najjar B.O, & Kandil Y.I. (2020). Hypolipidaemic and Insulin Secretagogue Activities of (R)-(−)-Carvone. The Malaysian Journal of Medical Sciences : MJMS, 27(6), 39-52.

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