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Thymidine 5 monophosphate p nitrophenyl ester

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Thymidine 5'-monophosphate p-nitrophenyl ester is a chemical compound used as a laboratory reagent. It serves as a substrate for various enzymatic reactions and assays.

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Lab products found in correlation

Bovine serum albumin (bsa), by Roche (1 mentions) P nitrophenol, by Merck Group (1 mentions)

Close spelling variants of this product

Sodium thymidine 5'-monophosphate p-nitrophenyl ester (nitrophenyl-pT), Thymidine 5′ – monophosphate p-nitrophenyl ester sodium salt P-nitrophenyl thymidine 5′-monophosphate Thymidine

3 protocols using thymidine 5 monophosphate p nitrophenyl ester

1

Enzymatic Assay for Thymidine Kinase Activity

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A day before the assay, cells were seeded at 5 x 104 cells per well in 96-well plates. Next day, culture media was replaced by 50 μl of the buffer assay (50 mM Tris, 250 mM NaCl, pH 9.5). Subsequently, 50 μL of the assay buffer containing the reagent Thymidine 5'-monophosphate p-nitrophenyl ester (Sigma, #T4510) at 10 mM diluted in deionized water was added in each well. Absorbance was measured at 405 nm over time. Measurements were taken after 60 minutes reaction.
Ruiz-Fernández de Córdoba B., Moreno H., Valencia K., Perurena N., Ruedas P., Walle T., Pezonaga-Torres A., Hinojosa J., Guruceaga E., Pineda-Lucena A., Abengózar-Muela M., Cochonneau D., Zandueta C., Martínez-Canarias S., Teijeira Á., Ajona D., Ortiz-Espinosa S., Morales X., Ortiz de Solórzano C., Santisteban M., Ramos-García L.I., Guembe L., Strnad V., Heymann D., Hervás-Stubbs S., Pío R., Rodríguez-Ruiz M.E., de Andrea C.E., Vicent S., Melero I., Lecanda F, & Martínez-Monge R. (2022). Tumor ENPP1 (CD203a)/Haptoglobin Axis Exploits Myeloid-Derived Suppressor Cells to Promote Post-Radiotherapy Local Recurrence in Breast Cancer. Cancer Discovery, 12(5), 1356-1377.
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2

Colorimetric Thymidine Phosphatase Assay

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A day before the assay, cells were seeded at 5 × 104 cells per well in 96-well plates. The next day, culture media were replaced by 50 µL of the buffer assay (50 mmol/L Tris, 250 mmol/L NaCl, pH 9.5). Subsequently, 50 µL of the assay buffer containing the reagent thymidine 5′-monophosphate p-nitrophenyl ester (Sigma, #T4510) at 10 mmol/L diluted in deionized water was added in each well. Absorbance was measured at 405 nm over time. Measurements were taken after 60-minute reaction.
Ruiz-Fernández de Córdoba B., Moreno H., Valencia K., Perurena N., Ruedas P., Walle T., Pezonaga-Torres A., Hinojosa J., Guruceaga E., Pineda-Lucena A., Abengózar-Muela M., Cochonneau D., Zandueta C., Martínez-Canarias S., Teijeira Á., Ajona D., Ortiz-Espinosa S., Morales X., Ortiz de Solórzano C., Santisteban M., Ramos-García L.I., Guembe L., Strnad V., Heymann D., Hervás-Stubbs S., Pío R., Rodríguez-Ruiz M.E., de Andrea C.E., Vicent S., Melero I., Lecanda F, & Martínez-Monge R. (2022). Tumor ENPP1 (CD203a)/Haptoglobin Axis Exploits Myeloid-Derived Suppressor Cells to Promote Post-Radiotherapy Local Recurrence in Breast Cancer. Cancer Discovery, 12(5), 1356-1377.
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3

Isolation and Characterization of Barley Malt Rootlets

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Dried barley (Hordeum distichum var. Rex) malt rootlets were kindly obtained as a by-product of the malting industry, "Slavonija-Slad" (Nova Gradiska, Croatia). Separation of malt rootlets from remains of hulls was achieved by manual sieving using a commercial circular sieve with 1 mm diameter holes. Thymidine 5'-monophosphate p-nitrophenyl ester (denoted as nitrophenyl-pT in this article) and p-nitrophenol were purchased from Sigma Chemical Co. (St. Louis, USA). Bovine serum albumin (BSA) and Tris base were purchased from Boehringer Mannheim (Germany). S-Sepharose and DEAE-Sepharose were purchased from Pharmacia AB (Sweden). Standard protein markers (low molecular weight) were purchased from Pharmacia Fine Chemicals (Sweden). All other reagents were of analytical grade.
Beluhan S., Karmelić I., & Šantek M.I. (2020). Adding value in barley malt rootlets as a source of 5’-phosphodiesterase. Hrvatski časopis za prehrambenu tehnologiju biotehnologiju i nutricionizam, 15(1-2), 27-37.
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