Bioraptr frd dispenser

A673 and MiaPaCa-2 cells were cultured as described above, and plated in 1536-well white solid tissue culture plates using a Multidrop Combi peristaltic dispenser (ThermoFisher, Waltham, MA) at a density of 500 cells/well in 5 μL of DMEM (Invitrogen 11965118) culture medium supplemented with 10 % fetal bovine serum and 100 units/mL Penicillin, 100 μg/mL Streptomycin. A 1536-well pintool dispenser outfitted with 20 nL pins (Wako Automation, San Diego, CA) was used to transfer 23 nL of compound in DMSO to the 1536-well assay plates. After 48 hr incubation at 37 °C, 2.5 μL of CellTiter-Glo (Promega) was dispensed into each well using a BioRAPTR FRD dispenser (Beckman Coulter, Brea, CA). Plates were incubated at room temperature for 10 minutes, transferred to a ViewLux microplate imager (PerkinElmer, Waltham, MA) and the ATP-coupled luminescence was measured using a 1 second exposure.

A673 and MiaPaCa-2 cells were cultured as described above, and plated in 1536-well black clear bottom tissue culture plates using a Multidrop Combi peristaltic dispenser (ThermoFisher, Waltham, MA) at a density of 500 cells/well in 4 μL of DMEM (Invitrogen 31053036) culture medium. A 1536-well pintool dispenser outfitted with 20 nL pins (Wako Automation, San Diego, CA) was used to transfer 23 nL of compound in DMSO to the 1536-well assay plates. After 2 hr incubation at 37 °C, 2 μL of reconstituted Lactate Reaction Mix (BioVision K607–100) was dispensed into each well using a BioRAPTR FRD dispenser (Beckman Coulter, Brea, CA). Plates were incubated at room temperature for 30 minutes, transferred to a ViewLux microplate imager (PerkinElmer, Waltham, MA) and the fluorescence (Ex/Em 525/598 nm) and absorbance (573 nm) were measured accordingly.