Tba 200fr chemical analyzer

Plasma glucose levels were measured by the glucose oxidase method using the TBA-200FR chemical analyzer (Toshiba, Tokyo, Japan). HbA1c levels were measured by ion-exchange high-performance liquid chromatography using HLC-723G8 (Tosoh, South San Francisco, CA, USA). C-peptide levels were measured using Modular Analytics E170 electrochemiluminescence immunoassays (Hitachi, Tokyo, Japan). Creatinine and ALT levels were measured using the TBA-200FR chemical analyzer, while eGFR was calculated using the Modification of Diet in Renal Disease equation [26 (link)]. For the quantification of iGLP-1 (GLP-1 (7–36) amide, GLP-1 (7–37)) and iGIP (GIP (1–42)), a measurement kit based on the principle of sandwich enzyme immunoassay (Code Nos. 27784 and 27201, respectively; Immuno-Biological Laboratories Co. Ltd., Gunma, Japan) was purchased and used. For the quantification of glucagon, a glucagon chemiluminescent kit based on sandwich enzyme-linked immunosorbent assay (Cat. No. EZGLU-30K, Merck KGaA, Darmstadt, Germany) was purchased and used.

Plasma glucose levels were measured by the glucose oxidase method using the TBA-200FR chemical analyzer (Toshiba, Tokyo, Japan). HbA1c levels were measured by ion-exchange high-performance liquid chromatography using HLC-723G8 (Tosoh, South San Francisco, CA, USA). C-peptide levels were measured using Modular Analytics E170 electrochemiluminescence immunoassays (Hitachi, Tokyo, Japan). Creatinine and ALT levels were measured using the TBA-200FR chemical analyzer, while eGFR was calculated using the Modification of Diet in Renal Disease equation. For the quantification of iGLP-1 (GLP-1 (7–36) amide, GLP-1 (7–37)) and iGIP (GIP (1–42)), a measurement kit based on the principle of sandwich enzyme immunoassay (Code Nos. 27784 and 27201, respectively; Immuno-Biological Laboratories Co. Ltd., Gunma, Japan) was purchased and used. For the quantification of glucagon, a glucagon chemiluminescent kit based on sandwich enzyme-linked immunosorbent assay (Cat. No. EZGLU-30K, Merck KGaA, Darmstadt, Germany) was purchased and used.