The extracts from Lisosan® G were centrifuged (5000 rpm, 10 min) and analyzed using a HP 1200L liquid chromatograph equipped with a DAD detector (Agilent Technologies, Palo Alto, CA, USA) using a Poroshell 120, EC-C18 column (150 × 3 mm, 2.7 µm, Agilent, USA). The solvents for the mobile phase were (A) CH3CN and (B) 0.1% formic acid/water; the multi-step linear solvent gradient was: 0–5 min 0–10% A; 5–10 min 10–15% A; 10–20 min 15–30% A; 20–25 min 30–35% A; 25–28 min 35–40% A; 28–31 min 40–45% A; 31–42 min 100% A; 42–47 min 100–0% A; equilibration time 5 min; flow rate 0.4 mL min−1; injection volume 10 μL. The following wavelengths were simultaneously selected: 240 nm, 280 nm, 330 nm, 350 nm.
Hp 1200l liquid chromatograph
Manufactured by Agilent Technologies
Sourced in United States
The HP 1200L liquid chromatograph is a high-performance liquid chromatography (HPLC) instrument designed for the separation, identification, and quantification of chemical compounds in liquid samples. It features a compact design, intuitive user interface, and advanced hardware and software components to enable precise and reliable analytical performance.
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2 protocols using hp 1200l liquid chromatograph
1
Lisosan® G Phytochemical Profiling
2
Analysis of Phenolic and Steryl Ferulates
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All the extracts were analyzed using a HP 1200L liquid chromatograph equipped with a DAD detector (Agilent Technologies, Palo Alto, CA, USA) with a Raptor ARC-18 column (150 × 3 mm, 5 µm, Restek, USA). The gradient method for the phenolic extracts was the same proposed by The HPLC-DAD-MS analyses of phenolic and steryl ferulates extracts were performed using the same column and chromatographic conditions previously described. HPLC-DAD-MS system was from Waters and composed by 2695 HPLC, 2996 PAD and 4 micro MS equipped with Zspray ESI source. The ESI interface parameters were capillary 3.50 kV, cone 76 V, source temperature 120 °C, desolvation gas temperature 350 °C, cone gas flow 25 (L/h), desolvation gas flow 370 (L/h). Data were acquired in negative ion mode from 150 m/z to 800 m/z, at 0.5 sec/scan rate. The chromatograms were recorded at 330 nm.
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