Fresh plant parts (stem and leaf) of mother as well as in vitro raised plants were washed under running tap water and blotted with tissue towel. The tissue was dried in room temperature. 100 mg of dried tissue was homogenized in 100 ml of respective solvent (methanol, chloroform, and acetone) and extractions were carried in an orbital shaker (REMI, India) with constant stirring at 150 rpm for 24 h. The mixtures were centrifuged at 10,000 rpm for 10 min and the supernatant was filtered through Whatman filter paper (No. 1). Measurements of biochemical parameters were taken on a Lambda-35 double beam spectrophotometer (Perkin-Elmer, USA).
Lambda 35 double beam spectrophotometer
Manufactured by PerkinElmer
Sourced in United States
The Lambda-35 double beam spectrophotometer is a laboratory instrument designed to measure the absorption or transmission of light in a sample. It features a dual-beam configuration, allowing for the simultaneous measurement of a sample and a reference. The instrument covers a wavelength range of 190 to 1100 nanometers and can be used for a variety of applications, including quantitative analysis, kinetics studies, and more.
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Lab products found in correlation
2 protocols using lambda 35 double beam spectrophotometer
1
Extraction of Plant Biomolecules
2
Synthesis and Characterization of Ruthenium(II) Complexes
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1,2-diaminoethane (en), (±)-trans-1,2-diamminocyclohexane (dach), 2,2'-bipyridine (bpy), 4'-chloro-2,2':6',2''-terpyridine (Cl-tpy), 4'-(4-chlorophenyl)-2,2':6',2''-terpyridine (Cl-Ph-tpy) and bovine serum albumin (BSA) are commercially available and were used as received. The complexes [Ru(Cl-tpy)(en)Cl]Cl (4), [Ru(Cl-tpy)(dach)Cl]Cl (5) and [Ru(Cltpy)(bpy)Cl]Cl (6) were synthesized as reported previously [6] . Microanalysis, UV-Vis spectra and 1 H NMR spectra were used to check the purity of these complexes and the spectra agreed well with the data already reported [6] . All other chemicals were used as purchased residual non-deuterated solvent (δ = 1.94). All NMR spectra were run at 298 K. The UV-Vis spectra were obtained on a Perkin-Elmer Lambda 35 double beam spectrophotometer, using 1.0 cm path-length quartz cuvettes (3.0 mL). Infrared spectra were recorded on a Perkin-Elmer 983G spectrometer. Fluorescence measurements were carried out on a RF-1501 PC spectrofluorometer (Shimadzu, Japan). The excitation and emission bandwidths were both 10 nm.
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