Eazi breed

Manufactured by Zoetis

Sourced in United States, Brazil, New Zealand

Eazi-Breed is a laboratory equipment product offered by Zoetis. It is designed for use in various scientific applications. The core function of Eazi-Breed is to facilitate efficient and controlled breeding processes, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

Lutalyse, by Zoetis (2 mentions) Lps escherichia coli o55 b5, by Merck Group (1 mentions) Factrel, by Zoetis (1 mentions) Hoboware, by Hobo (1 mentions) Estrumate, by MSD animal health (1 mentions) Receptal, by MSD animal health (1 mentions)

4 protocols using eazi breed

Immune Response of Heifers to LPS

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All procedures were approved by the New Mexico State University Institutional Animal Care and Use Committee (2017–030). Fourteen crossbred heifers (BW average = 416 kg) were housed and maintained at New Mexico State University (Las Cruces, NM).
Heifers were stratified by BW and assigned to vehicle-treated control (CON, n = 7) or LPS (n = 7) treatment groups. LPS (Escherichia coli O55:B5; Sigma–Aldrich, Inc., Milwaukee, WI) was prepared by dissolving 1 mg in 5 mL of sterile saline, then subcutaneously injected at a rate of 2.0 μg/kg of BW. Each animal received approximately 4 mL of solution on days 2, 5, and 8. Control animals were injected with sterile saline. Baseline rectal temperatures were taken 1 d before the start of the experiment to preclude any potential febrile response prior to administering treatment.
Heifers were synchronized to estrus using the Select Synch plus controlled internal drug release (CIDR) protocol. On day 0, a GnRH (2 mL, i.m., Factrel, Zoetis Inc. US) injection was administered, and a CIDR (Eazi-Breed, Zoetis Inc., Kalamazoo, MI) device was inserted. On day 7, the CIDR was removed, and heifers received PGF2á (Lutalyse, 25 mg, i.m., Zoetis Inc. US).
Rectal temperatures were taken just prior to treatment (30-Second Digital Thermometer, Target Corporation, Minneapolis, MN) and at 2, 3, 4, 8, and 10 h after injection.

Ferranti E.M., Aloqaily B.H., Gifford C.A., Löest C.A., Wenzel J.C, & Hernandez Gifford J.A. (2018). Lipopolysaccharide modulation of ovarian hormonal profile. Translational Animal Science, 2(Suppl 1), S31-S34.

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Monitoring Cow Thermal Stress and Physiology

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A humidity and temperature-measuring instrument (HOBO U23 Pro v2 Relative Temperature/Humidity Data Logger, Onset Computer Corp., Cape Cod, MA) was placed in a protected area closest to the bed or feed bunk in each pen, inside the barn. Temperature and humidity data were recorded every 10 min for 3 consecutive days. Software provided by the company (HOBOware, Onset Computer Corp.) was used to read the recorded temperature and humidity data. The temperature-humidity index (THI) was calculated following Mader et al. (2006) (link): THI = (0.8 × T°C) + [(RH/100) × (T°C -14.4)] + 46.4, where T = temperature and RH = relative humidity.
A temperature-recording data logger (Thermochron iButton, Lawrenceburg, KY) was coupled to an intravaginal progesterone implant (Eazi-Breed, Zoetis, São Paulo, Brazil), allowing the sensors to be in direct contact with the vaginal wall. The logger recorded vaginal temperature continuously every 10 min for 3 d (from to 9 to 11 d before timed AI (TAI).
Body condition score (1 to 5 scale in 0.25 increments; Wildman et al., 1982) (link) and milk production (AfiMilk MPC milk meter, Afikim, Israel) were recorded at the time of thermometer insertion.

Polsky L.B., Madureira A.M.L., Filho E.L.D., Soriano S., Sica A.F., Vasconcelos J.L.M, & Cerri R.L.A. (2017). Association between ambient temperature and humidity, vaginal temperature, and automatic activity monitoring on induced estrus in lactating cows. Journal of dairy science, 100(10).

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Ovulation Synchronization in Repeat-Breeder Cows

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The short-term 5-day P₄-PGF-GnRH-based programme of the present study was adapted from a previous study [18 (link)]. At the beginning of the hormonal protocol for ovulation synchronisation (day 0), a total of 60 selected repeat-breeder dairy cows with ovarian inactivity were implanted with an exogenous P₄-releasing device with 1.38 g of P₄ (controlled internal drug release (CIDR), Eazi-Breed; Zoetis Inc., Mahuhu Crescent, Auckland, New Zealand), concurrent with an injection of P₄ (50 mg; Steraloids, Inc., Newport, RI, USA), and CIDR inserts were removed from the vagina on day 5. On day 5, all dairy cows received a 250 µg dose (cloprostenol) of PGF (Estrumate, MSD Animal Health, Upper Hutt, Wellington, New Zealand). On day 8, a 10 µg dose (buserelin) of GnRH (Receptal, MSD Animal Health, Upper Hutt, Wellington, New Zealand) was administered concurrently with the FTAI in all dairy cows. The FTAI was performed on day 8 using a single dose of frozen-thawed semen. The completion of hormonal synchronisation period was designated as day 8.

Yama P., Yadmak C., Sangkate M., Jitjumnong J., U-krit W., Promsao N., Montha N., Sudwan P., Mektrirat R., Panatuk J., Inyawilert W., Sringarm K., Lumsangkul C., Tapingkae W., Doan H.V., Tang P.C, & Moonmanee T. (2022). In Vivo Follicular and Uterine Arterial Indices as an Indicator of Successful Hormonal Stimulation for Inactive Ovaries in Repeat-Breeder Crossbred Dairy Cows Using a Short-Term Progesterone-Based Programme. Animals : an Open Access Journal from MDPI, 12(3), 292.

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Synchronization and Breeding Protocol for Beef Heifers

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Estrus was synchronized using the 7-d CIDR-PG protocol. Heifers received a CIDR (controlled internal drug release device; Eazi-Breed, Zoetis Animal Health) insert for 7 d after which the CIDR was removed and all heifers received a single 5-mL i.m. injection of prostaglandin F2α (PGF2α) (Lutalyse, Zoetis Animal Health). At time of CIDR removal an estrus detection aid (Estrotect; MAI Animal Health, Elmwood, WI) was applied. Upon removal of CIDR insert, heifers were placed in a common pasture and estrus detection performed for 5 d following PGF2α administration. Heifers were subjected to artificial insemination (AI) approximately 12 h after observed standing estrus. Approximately 10 d following the last day of AI, heifers were exposed to bulls for approximately 45 d. First service conception rates were determined 30 d after AI and overall pregnancy rates were determined at a minimum of 30 d after bull removal by analyzing whole blood for pregnancy specific protein-B (Biopryn; Biotracking Inc., Moscow, ID).

Rosasco S.L., Kassetas C.J., Cox S.H., Dunlap R.L., Hernandez Gifford J.A., Scholljegerdes E.J, & Summers A.F. (2019). Effects of growth-promoting implants administered during the suckling phase or at weaning on growth and reproduction in replacement beef heifers grazing native range. Translational Animal Science, 3(Suppl 1), 1774-1778.

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