Qp2010 ultra mass

1 H NMR and 13 C NMR spectra were recorded with a Varian Mercury plus spectrometer at 400 MHz and 100 MHz. Sephadex LH-20 (Sigma-Aldrich) and silica gel (Kieselgel 60, 0.063-0.2 mm Merck 7734 and 0.040-0.063 mm Merck 9385 and LiChroprep RP-18, 25-40 m, Merck 9303) for column chromatography (CC); silica gel 60 F254 (Merck, 05554) for TLC were used. TLC spots were detected with a UV lamp and spraying 1% Vanillin/H2SO4 and heating at 120 °C for 1-2 min. Solvents used in isolation and solvent system are ethyl acetate, formic acid, methanol, H2SO4, H2O, HCl, hexane, chloroform (Merck), vanillin (Fluka). HPLC analysis was practiced using a Shimadzu liquid chromatography (Shimadzu Corporation, LC 20 AT, Kyoto, Japan) and C18 column (Zorbax, 4.6 mm x 150 mm, 5 µm particle size). GC analysis was carried out using a Shimadzu 2010 Plus gas chromatography coupled to a Shimadzu QP2010 Ultra mass selective detector.

The GC-MS analysis was performed using Shimadzu 2010 Plus gas chromatograph coupled to a Shimadzu QP2010 Ultra mass selective detector. The gas chromatography-flame ionization detector (GC-FID) was used. The fibre containing the extracted aroma compounds was injected into GC/MS injector. The split mode was used. The separation was performed by means of a Restek Rxi-5MS capillary column, 60 m length, 0.25 mm i.d., and a 0.25 μm phase thickness. The oven program was as follows: Initial temperature was 60 °C for 2 min, which was increased to 240 °C at 3 °C min -1 . 250 °C was maintained for 4 min. Helium (99.999%) was used as carrier gas with a constant flow-rate of 1 μL min -1 . Detection was carried out in electronic impact mode (EI) and ionization voltage was fixed to 70 eV. Scan mode (40-450 m/z) was used for mass acquisition. The volatile compounds were identified by comparison with the mass spectra of the two libraries (FFNSC1.2 and W9N11) and using the standard compounds (βpinene, camphene, limonene oxide, isopinocarveol, 1-terpinen-4-ol, β-ionone, caryophyllene oxide, biformene, and nonanal) (Renda et al., 2019) (link).