Forty-five 2-month-old male BALB/c.KOR/Stm Slc-Apoeshl mice (Japan SLC, Inc., Shizuoka, Japan) were kept in a room at a temperature of 22°C with a 12-hour light–dark cycle. The mice were administered either a high-fat diet (45% energy from fat, 24% fat content by weight, 4.73 kcal/g; D12451, Research Diet; Sankyo Labo Service Corporation, Inc., Japan) or a control diet (10% energy from fat, 4.3% fat content by weight, 3.85 kcal/g; D12450H, Research Diet), and drinking water was provided ad libitum.
All animal experiments followed the Institutional Guidelines of the Kanazawa Medical University (KMU) and the guiding principles of the Physiological Society of Japan. These experiments were approved by the Animal Care Committee of KMU and were performed at the Animal Care Center of KMU.
All animal experiments followed the Institutional Guidelines of the Kanazawa Medical University (KMU) and the guiding principles of the Physiological Society of Japan. These experiments were approved by the Animal Care Committee of KMU and were performed at the Animal Care Center of KMU.