Automated hematology analyzer kx 21n

On days 3, 6, and 9 after injection, rats were reweighed and glucose estimation was done in all the two (2) groups described above. On day 10, twenty-four (24) rats had very high plasma glucose level greater than 250 mg/dL and were included for group 2 while four (4) lower responsive rats were excluded. Animals were sacrificed by exposure to chloroform within a closed system and blood samples were collected for the various investigations into appropriate specimen bottles. The following investigations were carried out in the course of the study: haematocrit (HCT), haemoglobin (Hb), and red blood cell count (RBC), extracted from a complete blood count analysis using SYSMEX Automated Hematology Analyzer (KX-21N, Sysmex Corporation, Chuo-ku, Kobe 651-0073, Japan); peripheral blood for reticulocyte count incubated with new methylene blue at 37°C, smeared, and estimated manually; serum total bilirubin estimated using Randox kit (Randox Laboratories Ltd., BT29 QY, United Kingdom); calorimetric method on BSA 3000 Semiautometed Biochemistry Analyser (SFRI San, Lieu dit Berganton, 33127 Saint Jean d'lllac, France); and Plasma haemoglobin according to Dacie and Lewis [14 ]. Plasma potassium was estimated using ISE 6000 electrolyte analyzer (SFRI San, Lieu dit Berganton, 33127 Saint Jean d'lllac, France).

A total of 3.5 mL will be collected from the recruited subjects in step 2. The blood collected will be allowed to clot and centrifuged to produce the serum for biochemical analysis that will include serum TNF-α, IFN-γ, PCT, and CRP. The WBC counts and the differentials (polymorphonuclear cells) will be measured using the automated hematological analyzer (SYSMEX automated hematology analyzer KX-21N, Sysmex Corporation, Kobe, Japan). Serum levels of TNF-α and IFN-γ will be assayed by ELISA (BD Biosciences, United States) according to the manufacturer’s instructions. The TNF-α concentration will be calculated in the test samples on the basis of the curve produced by plotting the optical density values of the known standards (range: 7.5-500 pg/mL) on log-log graph paper.
The level of serum IFN-γ will also be detected with the IFN-γ ELISA kit. The detection limits of the kit for TNF-α and IFN-γ will be 2 and 1 pg/mL, respectively.
PCT will be measured with a quantitative immunoluminometric assay (LUMItest PCT, progressively replaced by PCT sensitive KRYPTOR, both from Brahms Diagnostica, Berlin, Germany), with a maximum interassay variation of approximately 0.3 ng/mL. CRP will be measured using the latex agglutination method and the automated method on Roche Integra 400 with an analytical goal of ±10%.