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Cellometer auto t4 counter

Manufactured by Revvity

The Cellometer Auto T4 counter is a compact and automated cell counting device. It utilizes the principle of fluorescence microscopy to accurately determine the total cell count and viability of cell samples. The instrument provides rapid and reliable results, making it a useful tool for various cell-based applications.

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3 protocols using cellometer auto t4 counter

1

Culturing and Differentiating Murine Brown Adipocytes

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The murine non-differentiated brown adipocyte cell line [18 (link)] (kindly provided by Dr A. Whittle, Institute of Metabolic Science, University of Cambridge, UK) was cultured in Dulbecco's Modified Eagle's Medium (DMEM) supplemented with 25 mM glucose, 10% fetal calf serum (FCS), 1% glutamine, 1% penicillin and 1% streptomycin in a humidified atmosphere at 37°C and 5% CO2. Differentiation of the brown adipocyte cell line into mature brown adipocytes was conducted as described [18 (link),19 (link)]. The density of non-differentiated brown adipocytes depending on the further experimentation was determined by the Cellometer Auto T4 counter (Nexcelom Bioscience).
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2

Cell Line Growth and Counting

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A total of 63 cell lines available at the NCFAD were seeded from frozen stocks and grown for 48 h at 37 °C and 5% CO2, except for Trichoplusia ni cells which were grown with shaking at 27 °C for 24 h before collection. Adherent cell lines were dissociated using 0.25% Trypsin-0.1% EDTA, and cells in suspension were spun down at 4 °C for 10 min at a relative centrifugal force of 600, and re-suspended in culture media. An aliquot of cells was stained with 0.2% trypan blue and counted using a Cellometer Auto T4 counter (Nexcelom Bioscience).
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3

Cell Culture Protocol for Diverse Cell Lines

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A total of 63 cell lines available at the NCFAD were seeded from frozen stocks and grown for 48 hours at 37°C and 5% CO 2 , except for Trichoplusia ni cells which were grown with shaking at 27°C for 24 hours before collection. Adherent cell lines were dissociated using 0.25% Trypsin-0.1% EDTA, and cells in suspension were spun down at 4°C for 10 minutes at a relative centrifugal force (rcf) of 600, and re-suspended in culture media. An aliquot of cells was stained with 0.2% trypan blue and counted using a Cellometer Auto T4 counter (Nexcelom Bioscience).
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